Sulfated polysaccharide from Gracilaria caudata reduces hypernociception and inflammatory response during arthritis in rodents
| Autor: | Luciano S. Chaves, Pedro Almir Feitosa Morais, Ana Lúcia Ponte Freitas, Renan Oliveira Silva Damasceno, Francisco Fábio Bezerra de Oliveira, Francisco Clark Nogueira Barros, André Luiz dos Reis Barbosa, Pedro Marcos Gomes Soares, Marcellus H.L.P. Souza, Poliana de Oliveira Cavalcante Alencar, Rudy D. Bingana, Jand Venes R. Medeiros, Samara Rodrigues Bonfim Damasceno Oliveira |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Freund's Adjuvant Anti-Inflammatory Agents Arthritis Inflammation Vascular permeability Rodentia 02 engineering and technology Pharmacology Biochemistry Nitric oxide Capillary Permeability 03 medical and health sciences chemistry.chemical_compound Mice Structural Biology Polysaccharides Edema medicine Animals Gracilaria Molecular Biology 030304 developmental biology 0303 health sciences biology Sulfates Zymosan Interleukin General Medicine 021001 nanoscience & nanotechnology medicine.disease Arthritis Experimental Immunohistochemistry Disease Models Animal chemistry Myeloperoxidase biology.protein medicine.symptom 0210 nano-technology Biomarkers |
| Zdroj: | International journal of biological macromolecules. 161 |
| ISSN: | 1879-0003 |
| Popis: | Polysaccharide from marine alga Gracilaria caudata has potential health benefits, such as anti-inflammatory, gastroprotective and antidiarrheal effects. Here, we investigated the effect of a sulfated polysaccharide from G. caudata (SP-GC) on hypernociception and inflammatory response in arthritis models. The animals received SP-GC (3, 10 or 30 mg/kg) 1 h before tibio-tarsal injection of zymosan. Hypernociception, histopathology, edema, vascular permeability, myeloperoxidase (MPO) activity, cell influx, interleukin (IL)-1β and nitric oxide (NO) levels were evaluated in acute phase. In another protocol, animals received SP-GC (30 mg/kg) 2 h post-complete Freund's adjuvant (CFA). Hypernociception, edema and arthritis index were determined in acute, sub-chronic and chronic phases. Rota-rod test measured the motor performance. SP-GC significantly reduced, in a dose-dependent manner, the zymosan-induced hypernociception with maximal effect at 30 mg/kg. The microscopic inflammation, joint edema, MPO activity, cell influx, IL-1β and NO levels were also reduced by SP-GC. In the CFA-induced arthritis, SP-GC inhibits the hypernociception, edema and arthritic index in acute, sub-chronic and chronic phases. SP-GC did not alter the motor performance of animals. In conclusion, SP-GC exerts protective effect in models of arthritis due to the modulation of cell influx, IL-1β and NO levels, culminating in the reduction of hypernociception and edema. |
| Databáze: | OpenAIRE |
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