COBAS® TaqMan® MTB, smear positivity grade and MGIT culture; correlation analyses of three methods for bacillary quantification
| Autor: | Hiroyuki Yamada, Satoshi Mitarai, Yuka Sasaki, Kiyohiko Izumi, Lina Yi, Akiko Takaki, Tomoko Kato, Akio Aono, Kinuyo Chikamatsu |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty Pathology Correlation coefficient Cobas taqman 030106 microbiology Statistics as Topic Real-Time Polymerase Chain Reaction Gastroenterology Sensitivity and Specificity Correlation Mycobacterium tuberculosis 03 medical and health sciences Internal medicine medicine TaqMan Humans Pharmacology (medical) Mycobacteria growth indicator tube Tuberculosis Pulmonary Rank correlation Bacteriological Techniques biology business.industry Sputum biology.organism_classification Infectious Diseases Evaluation Studies as Topic medicine.symptom business |
| Zdroj: | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 22(1) |
| ISSN: | 1437-7780 |
| Popis: | We investigated the correlation between the cycle threshold (Ct) value of the COBAS(®) TaqMan(®) MTB (TaqMan MTB), the mycobacterial smear positivity grade, and the time to detection (TTD) in the Mycobacteria Growth Indicator Tube (MGIT) for quantification of Mycobacterium tuberculosis (MTB). For 57 sputum samples, significant correlations were observed between the Ct value, the smear positivity grade, and the MGIT TTD (Spearman's rank correlation coefficient: r(s) = -0.940, P < 0.001 and Pearson's correlation coefficient: r(p) = 0.737, P < 0.001). In addition, a correlation was observed between the number of bacteria estimated based on the smear positivity grade and the number of MTB bacilli calculated by the Ct value (r(s) = 0.930, P < 0.001). This study has demonstrated the possible estimation of the smear positivity grade and MGIT TTD using the Ct value of TaqMan MTB, which is based on a real-time PCR system, for diagnostic samples. |
| Databáze: | OpenAIRE |
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