BIOLOGICAL ACTIVITY OF YERSINIA PSEUDOTUBERCULOSIS TOXINS
| Autor: | Rasskazov Va, Timchenko Nf, Terentieva Na, Golotin Va |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures nucleoside-kinases protein biosynthesis Medicine (miscellaneous) dna Biology Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Biosynthesis biology.animal Protein biosynthesis Yersinia pseudotuberculosis rna 030212 general & internal medicine Sea urchin chemistry.chemical_classification urogenital system toxins Embryo General Medicine biology.organism_classification QR1-502 Amino acid 030104 developmental biology Enzyme chemistry Biochemistry yersinia pseudotuberculosis embryonic structures Nucleic acid embryos of strongylocentrotus intermedius sea urchin |
| Zdroj: | Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 6, Pp 10-19 (2016) |
| ISSN: | 2686-7613 0372-9311 |
| Popis: | Aim. Study of effect of heat-labile (HLT) and thermostable (HST) lethal toxins of Yersinia pseudotuberculosis on the development of embryos of sea urchin Strongylocentrotus intermedius, processes of biosynthesis of nucleic acids and protein in embryo cells and activity of nucleoside-kinases of sea urchin. Materials and methods. Y. pseudotuberculosis strains 2517 (pYV-) and 512 (pYV48MD, pYV82MD) were used for isolation of HLT and HST. Gametes and embryos of sea urchin S. intermedius were used to carry out the experiments and isolate nucleoside-kinases. Results. Both of the studied toxins of Y. pseudotuberculosis possessed spermiotoxic effect and reduced fertilizing ability of sea urchin spermies. HLT LD50 was 1 (ig/ml, and HST - 2 pg/ml. Toxins affected the development of embryos of sea urchin resulting in severe morphologic damages, cessation of the development of embryos at early stages of embryogenesis, destruction of cells and death of embryos. Wherein, damaging effect of HLT was observed at lower concentrations compared with HST. HLT inhibited DNA and RNA biosynthesis at concentrations of 1-2 pg/ml. HST did not affect biosynthesis of nucleic acids even at high concentrations, but inhibited protein biosynthesis in sea urchin embryos. HLT did not reduce the level of inclusion of labeled amino acids into embryo cells. HLT had inhibiting effect on the activity of thymidine- and uridine-kinase of sea urchin, whereas HST did not affect these enzymes. Conclusion. Both of Y. pseudotuberculosis protein toxins affect the development of sea urchin embryos, however, mechanisms of action of HLT and HST on embryos and processes occurring in them differ. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|