Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR–Cas9 System
| Autor: | Ari E. Friedland, Monica P. Colaiácovo, George M. Church, Saravanapriah Nadarajan, John A. Calarco, Yonatan B Tzur |
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| Rok vydání: | 2013 |
| Předmět: |
Genetics
Genome Helminth biology Gene targeting Mutagenesis (molecular biology technique) DNA Helminth biology.organism_classification Germline Mutagenesis Insertional Genome editing Mutagenesis RNA editing Notes Gene Targeting Animals CRISPR DNA Breaks Double-Stranded RNA Editing CRISPR-Cas Systems Caenorhabditis elegans Genetic Engineering Homologous Recombination Homologous recombination |
| Zdroj: | Genetics. 195:1181-1185 |
| ISSN: | 1943-2631 |
| Popis: | We adapted the CRISPR–Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans. |
| Databáze: | OpenAIRE |
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