Homozygosity Mapping Reveals Population History and Trait Architecture in Self-Incompatible Pear (
Autor: | Satish Kumar, Cecilia Hong Deng, Daryl D. Rowan, Martin B. Hunt, Chris Kirk, Jun Wu, Lester Brewer, Claudia Wiedow |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Population inbreeding Locus (genetics) Plant Science Runs of Homozygosity Biology Quantitative trait locus lcsh:Plant culture 01 natural sciences Pyrus 03 medical and health sciences genotyping-by-sequencing lcsh:SB1-1110 education genome wide association study Original Research Genetics education.field_of_study runs of homozygosity signatures of selection germplasm Genetic architecture 030104 developmental biology quantitative trait loci Trait Inbreeding 010606 plant biology & botany |
Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 11 (2021) |
ISSN: | 1664-462X |
Popis: | Runs of homozygosity (ROH) have been widely used to study population history and trait architecture in humans and livestock species, but their application in self-incompatible plants has not been reported. The distributions of ROH in 199 accessions representing Asian pears (45), European pears (109), and interspecific hybrids (45) were investigated using genotyping-by-sequencing in this study. Fruit phenotypes including fruit weight, firmness, Brix, titratable acidity, and flavor volatiles were measured for genotype-phenotype analyses. The average number of ROH and the average total genomic length of ROH were 6 and 11 Mb, respectively, in Asian accessions, and 13 and 30 Mb, respectively, in European accessions. Significant associations between genomic inbreeding coefficients (FROH) and phenotypes were observed for 23 out of 32 traits analyzed. An overlap between ROH islands and significant markers from genome-wide association analyses was observed. Previously published quantitative trait loci for fruit traits and disease resistances also overlapped with some of the ROH islands. A prominent ROH island at the bottom of linkage group 17 overlapped with a recombination-supressed genomic region harboring the self-incompatibility locus. The observed ROH patterns suggested that systematic breeding of European pears would have started earlier than of Asian pears. Our research suggest that FROH would serve as a novel tool for managing inbreeding in gene-banks of self-incompatible plant species. ROH mapping provides a complementary strategy to unravel the genetic architecture of complex traits, and to evaluate differential selection in outbred plants. This seminal work would provide foundation for the ROH research in self-incompatible plants. |
Databáze: | OpenAIRE |
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