Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuoles
| Autor: | Garcia M, Soulet C, Elías-Villalobos A, María Moriel-Carretero, Sylvain Kumanski |
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| Přispěvatelé: | Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Centre de recherche de l'Institut Curie [Paris], Institut Curie [Paris] |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
biology Chemistry [SDV]Life Sciences [q-bio] Saccharomyces cerevisiae Autophagy Vacuole Ingression biology.organism_classification Cell biology 03 medical and health sciences Metabolic pathway 0302 clinical medicine medicine.anatomical_structure Cytoplasm Organelle medicine Nucleus 030217 neurology & neurosurgery 030304 developmental biology |
| Popis: | Membrane contact sites are functional nodes at which organelles exchange information through moving ions, proteins and lipids, thus driving the reorganization of metabolic pathways and the adaptation to changing cues. The nuclear-vacuole junction of Saccharomyces cerevisiae is among the most extensive and better-known organelle contact sites, described to expand in response to various metabolic stresses. While using genotoxins with unrelated purposes, we serendipitously discovered a phenomenon that we describe as the most extreme and intimate contact ever reported between nuclei and vacuoles: the vacuole becomes completely internalized in the nucleus. We define lipids supporting negative curvature, such as phosphatidic acid and sterols, as bona-fide drivers of this event. Functionally, we purport that internalized vacuoles are low efficiency ones whose removal from the cytoplasm optimizes cargo interaction with functional vacuoles. Thus, our findings also point to nucleus-vacuole interactions as important for metabolic adaptation. Yet, rather than by inter-organelle exchanges, the underlying mechanism literally concurs with vacuolar sequestration. |
| Databáze: | OpenAIRE |
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