DNA synthesis during bacterial conjugation
| Autor: | Obaid Siddiqi, P. Vijay Sarathy |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
biology
DNA synthesis DNA polymerase DNA polymerase II Circular bacterial chromosome DNA replication Eukaryotic DNA replication Merozygote biochemical phenomena metabolism and nutrition Origin of replication Molecular biology DnaA carbohydrates (lipids) Replication factor C Control of chromosome duplication Structural Biology biology.protein bacteria Replication protein A Molecular Biology S phase |
| Zdroj: | Journal of Molecular Biology. 78:443-451 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(73)90467-1 |
| Popis: | Conjugation in Escherichia coli involves an oriented transfer of DNA from the Hfr to the F−. We have examined the course of DNA replication in a donor cell while it is transferring its DNA. Using isotopic density shift for estimating replication, we have shown that mating is accompanied by initiation of a new round of DNA replication in Hfr cells. With the onset of F-mediated transfer replication, the normal vegetative replication in the Hfr appears to be suppressed. Experiments with F′ donors indicate that the transfer of the chromosome is necessary for switching off vegetative replication. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|