Optotheranostic Nanosystem with Phone Visual Diagnosis and Optogenetic Microbial Therapy for Ulcerative Colitis At-Home Care
| Autor: | Shufang Zhang, Jin Chang, Chun Yang, Cui Meihui, Hanjie Wang, Gaoju Pang, Huizhuo Pan, Jing Liu, Ruru Kang, Lili Zhang, Tao Zhang, Xinyu Zhang, Xin Xue, Tao Sun |
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| Rok vydání: | 2021 |
| Předmět: |
Inflammatory response
General Physics and Astronomy Inflammation 02 engineering and technology Disease Optogenetics 010402 general chemistry 01 natural sciences Mice Health services Intestinal mucosa Animals Humans Medicine General Materials Science Intestinal Mucosa Recombinant escherichia coli business.industry General Engineering 021001 nanoscience & nanotechnology medicine.disease Home Care Services Ulcerative colitis 0104 chemical sciences Intestines Immunology Colitis Ulcerative medicine.symptom 0210 nano-technology business |
| Zdroj: | ACS Nano. 15:7040-7052 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.1c00135 |
| Popis: | Ulcerative colitis (UC) is a relapsing disorder characterized by chronic inflammation of the intestinal tract. However, the home care of UC based on remote monitoring, due to the operational complexity and time-consuming procedure, restrain its widespread applications. Here we constructed an optotheranostic nanosystem for self-diagnosis and long-acting mitigations of UC at home. The system included two major modules: (i) A disease prescreening module mediated by smartphone optical sensing. (ii) Disease real-time intervention module mediated by an optogenetic engineered bacteria system. Recombinant Escherichia coli Nissle 1917 (EcN) secreted interleukin-10 (IL-10) could downregulate inflammatory cascades and matrix metalloproteinases; it is a candidate for use in the therapeutic intervention of UC. The results showed that the Detector was able to analyze, report, and share the detection results in less than 1 min, and the limit of detection was 15 ng·mL-1. Besides, the IL-10-secreting EcN treatment suppressed the intestinal inflammatory response in UC mice and protected the intestinal mucosa against injury. The optotheranostic nanosystems enabled solutions to diagnose and treat disease at home, which promotes a mobile health service development. |
| Databáze: | OpenAIRE |
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