Analysis of the effects of different nitrogen sources and calcium on the production of amphotericin by Streptomyces nodosus based on comparative transcriptome
| Autor: | Kai Huang, Zhiqiang Liu, Bo Zhang, Yu-Guo Zheng, Chen Yu, Zhe-Ming Wu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antifungal Agents
Nitrogen Biomedical Engineering Antifungal drug Bioengineering Citrate (si)-Synthase Applied Microbiology and Biotechnology Transcriptome Streptomyces nodosus Amphotericin B Drug Discovery Gene cluster Citrate synthase Animals Pyruvates biology Chemistry Process Chemistry and Technology General Medicine biology.organism_classification Pyruvate dehydrogenase complex Streptomyces Metabolic pathway Biochemistry biology.protein Molecular Medicine Fermentation Calcium Cattle Oxidoreductases Biotechnology |
| Zdroj: | Biotechnology and applied biochemistryREFERENCES. 69(4) |
| ISSN: | 1470-8744 |
| Popis: | Streptomyces nodosus is known as the main manufacturer of amphotericin B, which is an effective antifungal drug. It is verified that the optimization of fermentation conditions and key growth factors have a great impact on the yield of AmB. The AmB production of S. nodosus in cottonseed-meal medium was 1.6 times than that of beef-paste medium. The transcriptome analysis was used to analyze the effects of different nitrogen media and calcium on S. nodosus. Related genes of the EMP and TCA pathways, such as phosphofructokinase, pyruvate dehydrogenase and citrate synthase, were up-regulated in cottonseed-meal medium. The expression level of the PKS modules of the amphotericin synthesis gene cluster in beef-paste medium was higher. Other functional genes, such as amphGH and amphRIV, have the advantage of expressing in cottonseed-meal medium. Ca2+ promoted the up-regulation of genes in metabolic pathways such as EMP pathway (pyruvate dehydrogenase), TCA pathway (citrate synthase) and amphotericin synthesis genes (PKS modules). The expression of WhiB family genes SNOD_RS 13310 and SNOD_RS 17625 was positively correlated with Ca2+ concentration. In addition, in the presence of calcium, the expression level of Sec transport system genes of S. nodosus was lower. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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