Utility of saliva and hair follicles in donor selection for hematopoietic stem cell transplantation and chimerism monitoring
| Autor: | Shekhar C. Neolia, Gurvinder Kaur, Sunil Kumar, Mrinali Hakim, Ramya Nandakumar, Jasmeet Kaur, Prateek Mahalwar, Abhinav Garg, Narinder K. Mehra, Chowphi C. Rapthap, Heena Kumra, Neeraj Kumar, Gaurav Sharma, Lalit Kumar |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent medicine.medical_treatment Human leukocyte antigen Hematopoietic stem cell transplantation Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry Chimerism Organ transplantation Donor Selection Young Adult hemic and lymphatic diseases Genetics medicine Humans Molecular Biology Alleles Electrophoresis Agar Gel saliva HLA-A Antigens Donor selection Hematopoietic Stem Cell Transplantation hair DNA Phlebotomy Middle Aged Hair follicle DNA Fingerprinting Transplantation HLA medicine.anatomical_structure surgical procedures operative DNA profiling Tandem Repeat Sequences Immunology Female Hair Follicle Research Paper transplantation |
| Zdroj: | Chimerism |
| ISSN: | 1938-1964 1938-1956 |
| Popis: | Selection of an HLA identical donor is a critical pre-requisite for successful hematopoietic stem cell transplantation (HSCT). Most transplant centers utilize blood as the most common source of DNA for HLA testing. However, obtaining blood through phlebotomy is often challenging in patients with conditions like severe leucopenia or hemophilia, pediatric and elderly patients. We have used a simple in-house protocol and shown that HLA genotypes obtained on DNA extracted from saliva or hair are concordant with blood and hence can be used for selection of donors for HSCT or organ transplantation. Similarly, for post-HSCT chimerism monitoring, non-availability of pre-transplant DNA samples poses a major limitation of reference STR fingerprints. This study shows that DNA obtained post-HSCT from hair follicles can be used to generate pre-transplant patient specific fingerprints while the STR profiles obtained in saliva samples cannot as these display a mixed state of chimerism. |
| Databáze: | OpenAIRE |
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