First report and molecular characterization of the dagger nematode, Xiphinema oxycaudatum (Nematoda, Dorylaimidae) from South Africa
Autor: | Antoinette Swart, Rinus Knoetze, Fisayo Daramola, Antoinette P. Malan |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Dorylaimidae Agriculture and Forestry Nematoda Short Communication Dorylaimida coxI Adenophorea molecular identification 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Nemata Plant virus Xiphinema lcsh:Zoology Botany Biodiversity & Conservation D2D3 Animalia lcsh:QL1-991 Ecology Evolution Behavior and Systematics Dorylaimoidea Soil health Sphaeronematidae Rhizosphere biology Phylogenetic tree Cenozoic Margollus Cephalornis biology.organism_classification 030104 developmental biology Xiphinema americanum Nematode Africa Dorylaimia Animal Science and Zoology honeybush Monoculture |
Zdroj: | ZooKeys, Vol 894, Iss, Pp 1-17 (2019) ZooKeys |
ISSN: | 1313-2970 1313-2989 |
Popis: | Plant-parasitic nematodes of the genus Xiphinema Cobb, 1913 comprise a complex group of nematode species, some of which are important vectors of plant viruses. During a field survey to determine the soil health of an abandoned honeybush (Cyclopia genistoides) monoculture, a high density of the dagger nematode, Xiphinema oxycaudatum Lamberti & Bleve-Zacheo, 1979 (Nematoda, Dorylaimidae), was observed in soil around the roots of honeybush plants in an abandoned farmland at Bereaville, an old mission station in the Western Cape province of South Africa. Soil samples were taken from the rhizosphere of plants and nematodes were extracted from the soil using a modified extraction tray method. Specimen of the dagger nematodes were processed for scanning electron microscopy, morphological and molecular analysis. Molecular profiling of the nematode species was done in order to give an accurate diagnosis and to effectively discriminate the nematode from other species within the Xiphinema americanum group. Phylogenetic analysis based on the D2D3 expansion segment of the 28S gene supported a close relationship of species within the americanum group, however, the protein-coding cytochrome oxidase (coxI) of the mitochondrial gene provided a useful tool for distinguishing the nematode from other species within the group. This study represents the first report of X. oxycaudatum from South Africa. |
Databáze: | OpenAIRE |
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