PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: Application to isolates and stool samples
| Autor: | J. M. J. Logan, John Stanley, Louise A. Metherell |
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| Předmět: |
Microbiology (medical)
Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Microtiter plate Feces Species Specificity law RNA Ribosomal 16S Campylobacter Infections medicine Humans Ribosomal DNA Polymerase chain reaction Hybridization probe Campylobacter Genes rRNA Bacteriology Amplicon Reference Standards 16S ribosomal RNA biology.organism_classification Molecular biology Bacterial Typing Techniques Campylobacter coli DNA Probes |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| Popis: | We report a PCR–enzyme-linked immunosorbent assay which identifies Campylobacter species by capture hybridization of a single-stranded 16S rRNA gene amplicon with species-specific probes in a microtiter plate format. Specificities were confirmed for both reference and field strains, but the type strain of Campylobacter coli was atypical. The assay was rapid, informative, and usable with stool-extracted DNA. |
| Databáze: | OpenAIRE |
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