Anti-Cancer Activity of Lobaric Acid and Lobarstin Extracted from the Antarctic Lichen Stereocaulon alpnum
| Autor: | Tai Kyoung Kim, Ju-Mi Hong, Sung-Suk Suh, Ui Joung Youn, Se Jong Han, Il-Chan Kim, Joung Han Yim, Jung Eun Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Uterine Cervical Neoplasms Pharmaceutical Science Analytical Chemistry HeLa Lactones lobaric acid 0302 clinical medicine human cervix adenocarcinoma Drug Discovery Hydroxybenzoates Molecular Structure biology Apoptosis Regulator Chemistry apoptosis Cell cycle Salicylates Gene Expression Regulation Neoplastic Proto-Oncogene Proteins c-bcl-2 Chemistry (miscellaneous) cell cycle arrest 030220 oncology & carcinogenesis Colonic Neoplasms Molecular Medicine Female human colon carcinoma Poly(ADP-ribose) Polymerases antarctic lichen Lichens lobarstin Cell Survival Poly ADP ribose polymerase Antineoplastic Agents Depsides Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Humans Physical and Theoretical Chemistry Benzofurans Cell Proliferation Dose-Response Relationship Drug Cell growth Organic Chemistry Cell Cycle Checkpoints HCT116 Cells biology.organism_classification 030104 developmental biology Cell culture Apoptosis Cancer research Drug Screening Assays Antitumor HeLa Cells |
| Zdroj: | Molecules; Volume 23; Issue 3; Pages: 658 Molecules, Vol 23, Iss 3, p 658 (2018) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules23030658 |
| Popis: | Lobaric acid and lobarstin, secondary metabolites derived from the antarctic lichen Stereocaulon alpnum, exert various biological activities, including antitumor, anti-proliferation, anti-inflammation, and antioxidant activities. However, the underlying mechanisms of these effects have not yet been elucidated in human cervix adenocarcinoma and human colon carcinoma. In the present study, we evaluated the anticancer effects of lobaric acid and lobarstin on human cervix adenocarcinoma HeLa cells and colon carcinoma HCT116 cells. We show that the proliferation of Hela and HCT116 cells treated with lobaric acid and lobarstin significantly decreased in a dose- and time-dependent manner. Using flow cytometry analysis, we observed that the treatment with these compounds resulted in significant apoptosis in both cell lines, following cell cycle perturbation and arrest in G2/M phase. Furthermore, using immunoblot analysis, we investigated the expression of cell cycle and apoptosis-related marker genes and found a significant downregulation of the apoptosis regulator B-cell lymphoma 2 (Bcl-2) and upregulation of the cleaved form of the poly (ADP-ribose) polymerase (PARP), a DNA repair and apoptosis regulator. These results suggest that lobaric acid and lobarstin could significantly inhibit cell proliferation through cell cycle arrest and induction of apoptosis via the mitochondrial apoptotic pathway in cervix adenocarcinoma and colon carcinoma cells. Taken together, our data suggests that lobaric acid and lobarstin might be novel agents for clinical treatment of cervix adenocarcinoma and colon carcinoma. |
| Databáze: | OpenAIRE |
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