Monoclonal Antibodies Recognizing Early and Late Antigens of Human Cytomegalovirus: Heterogeneity of Polypeptides Recognized between Virus Isolates
| Autor: | J. G. P. Sissons, J. Mundin, B. C. Rodgers |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Human cytomegalovirus
medicine.drug_class Immunoprecipitation viruses Cytomegalovirus Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Biology Monoclonal antibody Immunofluorescence Virus Antigen Virology medicine Humans Antigens Viral Immunoassay medicine.diagnostic_test Antibodies Monoclonal virus diseases biochemical phenomena metabolism and nutrition medicine.disease Molecular biology Molecular Weight Blot Cytomegalovirus Infections biology.protein Antibody |
| Zdroj: | Journal of General Virology. 66:2045-2049 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-66-9-2045 |
| Popis: | Summary The characteristics of four human cytomegalovirus (HCMV)-specific monoclonal antibodies as assessed by ELISA, immunofluorescence, immunoprecipitation and Western blotting are described. Two antibodies recognized a 67K late polypeptide of HCMV, one recognized 43K and 79K polypeptides present early and late in HCMV-infected cells, and the fourth identified a 72K early nuclear protein of HCMV. The antibodies recognized these antigens in all HCMV isolates tested by immunofluorescence and ELISA, but demonstrated inter-isolate variations in polypeptides recognized by Western blotting. |
| Databáze: | OpenAIRE |
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