STR-33, a Novel G Protein-coupled Receptor That Regulates Locomotion and Egg Laying in Caenorhabditis elegans
| Autor: | Hyoe Jin Joo, Jeong Eui Lee, Hyeon Sook Koo, Tae Hoon Lee, Heekyeong Kim, Young Ki Paik, Pan Young Jeong |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Serotonin
G protein Mutant GTP-Binding Protein alpha Subunits Gi-Go Biochemistry Receptors G-Protein-Coupled Neurotransmitter receptor Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Receptor Molecular Biology G protein-coupled receptor Neurons biology Reproduction Cell Biology biology.organism_classification Molecular biology Acetylcholine Receptors Neurotransmitter Cell biology Gq alpha subunit Mutagenesis Mutation embryonic structures biology.protein GTP-Binding Protein alpha Subunits Gq-G11 Female Signal transduction Locomotion Developmental Biology |
| Zdroj: | Journal of Biological Chemistry. 286:39860-39870 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m111.241000 |
| Popis: | Despite their predicted functional importance, most G protein-coupled receptors (GPCRs) in Caenorhabditis elegans have remained largely uncharacterized. Here, we focused on one GPCR, STR-33, encoded by the str-33 gene, which was discovered through a ligand-based screening procedure. To characterize STR-33 function, we performed UV-trimethylpsolaren mutagenesis and isolated an str-33-null mutant. The resulting mutant showed hypersinusoidal movement and a hyperactive egg-laying phenotype. Two types of egg laying-related mutations have been characterized: egg laying-deficient (Egl-d) and hyperactive egg laying (Egl-c). The defect responsible for the egg laying-deficient Egl-d phenotype is related to Gα(q) signaling, whereas that responsible for the opposite, hyperactive egg-laying Egl-c phenotype is related to Gα(o) signaling. We found that the hyperactive egg-laying defect of the str-33(ykp001) mutant is dependent on the G protein GOA-1/Gα(o). Endogenous acetylcholine suppressed egg laying in C. elegans via a Gα(o)-signaling pathway by inhibiting serotonin biosynthesis or release from the hermaphrodite-specific neuron. Consistent with this, in vivo expression of the serotonin biosynthetic enzyme, TPH-1, was up-regulated in the str-33(ykp001) mutant. Taken together, these results suggest that the GPCR, STR-33, may be one of the neurotransmitter receptors that regulates locomotion and egg laying in C. elegans. |
| Databáze: | OpenAIRE |
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