In vivo production of pederin by labrenzin pathway expansion
| Autor: | de la Calle F, José Luis García, Librada M. Cañedo, Beatriz Galán, Dina Kačar, Carmen Cuevas, Pilar Rodríguez, Carmen Schleissner |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Kacar, Dina [0000-0002-0141-0357], Schleissner, Carmen [0000-0003-2979-7140], Cañedo, Librada M. [0000-0002-1273-4138], De la Calle, Fernando [0000-0001-8161-8299], Galán, Beatriz [0000-0002-2596-6034], García, José Luis [0000-0002-9238-2485], Kacar, Dina, Schleissner, Carmen, Cañedo, Librada M., De la Calle, Fernando, Galán, Beatriz, García, José Luis |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2214-0301 |
| DOI: | 10.1016/j.mec.2022.e00198 |
| Popis: | 5 p.-3 fig. Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus Paederus. Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynthetic genes responsible for the production of pederin, it has not yet been possible to isolate and cultivate its bacterial endosymbiont producer. However, the marine bacterium Labrenzia sp. PHM005 was recently reported to produce labrenzin, the closest pederin analog. By cloning a synthetic pedO gene encoding one of the three O-methyltraferase of the pederin cluster into Labrenzia sp. PHM005 we have been able to produce pederin for the first time by fermentation in the new recombinant strain. The present study was funded by the Ministry of Economy and Competitiveness of Spain under the program Retos/Colaboración with the project number RTC-2016-4892-1 (DESPOL). Biotecnología aplicada al Desilenciamiento génico de Policétidos y Péptidos no ribosomales bioactivos en bacterias marinas. DESPOL |
| Databáze: | OpenAIRE |
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