Neuroprotective Effects of Clostridium butyricum against Vascular Dementia in Mice via Metabolic Butyrate
| Autor: | Junjie Yu, Haixiao Li, Zongxin Ling, Jiru Xu, Wenqian Chen, Mengqi Pang, Jiaming Liu, Jiangtao Jin, Xichong Yu, Huiqing Zhang, Jing Sun, Yiwen He, Fangyan Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Article Subject lcsh:Medicine Apoptosis Butyrate Gut flora Pharmacology Hippocampus Neuroprotection General Biochemistry Genetics and Molecular Biology Mice Phosphatidylinositol 3-Kinases Western blot medicine Animals Phosphorylation Vascular dementia Protein kinase B Clostridium butyricum Brain-derived neurotrophic factor Mice Inbred ICR General Immunology and Microbiology biology medicine.diagnostic_test Brain-Derived Neurotrophic Factor Dementia Vascular Probiotics lcsh:R Brain General Medicine medicine.disease biology.organism_classification Immunohistochemistry Butyrates Disease Models Animal Microscopy Electron Carotid Arteries Neuroprotective Agents Immunology Research Article |
| Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2015/412946 |
| Popis: | Probiotics actively participate in neuropsychiatric disorders. However, the role of gut microbiota in brain disorders and vascular dementia (VaD) remains unclear. We used a mouse model of VaD induced by a permanent right unilateral common carotid arteries occlusion (rUCCAO) to investigate the neuroprotective effects and possible underlying mechanisms ofClostridium butyricum. Following rUCCAO,C. butyricumwas intragastrically administered for 6 successive weeks. Cognitive function was estimated. Morphological examination was performed by electron microscopy and hematoxylin-eosin (H&E) staining. The BDNF-PI3K/Akt pathway-related proteins were assessed by western blot and immunohistochemistry. The diversity of gut microbiota and the levels of butyrate in the feces and the brains were determined. The results showed thatC. butyricumsignificantly attenuated the cognitive dysfunction and histopathological changes in VaD mice.C. butyricumnot only increased the levels of BDNF and Bcl-2 and decreased level of Bax but also induced Akt phosphorylation (p-Akt) and ultimately reduced neuronal apoptosis. Moreover,C. butyricumcould regulate the gut microbiota and restore the butyrate content in the feces and the brains. These results suggest thatC. butyricummight be effective in the treatment of VaD by regulating the gut-brain axis and that it can be considered a new therapeutic strategy against VaD. |
| Databáze: | OpenAIRE |
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