Linezolid Resistance in SequentialStaphylococcus aureusIsolates Associated with a T2500A Mutation in the 23S rRNA Gene and Loss of a Single Copy of rRNA
| Autor: | George M. Eliopoulos, Venkata G. Meka, Robert C. Moellering, Howard S. Gold, Satish K. Pillai, George Sakoulas, Christine Wennersten, Lata Venkataraman, Paola C. DeGirolami |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Staphylococcus aureus Gene Dosage Microbial Sensitivity Tests Drug resistance Biology medicine.disease_cause DNA Ribosomal Microbiology Minimum inhibitory concentration chemistry.chemical_compound Anti-Infective Agents 23S ribosomal RNA Acetamides Drug Resistance Bacterial medicine Humans Point Mutation Immunology and Allergy Ribosomal DNA Oxazolidinones Antibacterial agent Linezolid Genes rRNA Sequence Analysis DNA Chromosomes Bacterial Staphylococcal Infections biochemical phenomena metabolism and nutrition Ribosomal RNA bacterial infections and mycoses Virology Blotting Southern RNA Bacterial RNA Ribosomal 23S Blood Infectious Diseases chemistry Mutation Methicillin Resistance Gene Deletion |
| Zdroj: | The Journal of Infectious Diseases. 190:311-317 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/421471 |
| Popis: | Linezolid is an important therapeutic option for infections caused by resistant gram-positive bacteria. We report the characterization of sequential methicillin-resistant Staphylococcus aureus (MRSA) bloodstream isolates that developed resistance in a patient treated with a prolonged course of linezolid. Analysis of this series of clinical MRSA isolates detected, in the resistant isolates, the presence of a T2500A mutation in the domain V region of the 23S rRNA gene. In addition, the loss of a single copy of the 23S rRNA gene was found in 2 of the resistant isolates. As a result of these 2 factors, the proportion of mutant : wild-type 23S rRNA genes increased in association with an increase in the minimum inhibitory concentration of linezolid. The most recent isolate of this series was recovered 7 months after the patient discontinued linezolid and demonstrated reversion to a susceptible phenotype associated with a loss of the T2500A mutation. |
| Databáze: | OpenAIRE |
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