UVA-photoprotective potential of silymarin and silybin

Autor: Alena Ryšavá, Jitka Ulrichová, Pavel Kosina, Eva Gabrielová, Loizos Michaelides, Jitka Vostálová, Alena Rajnochová Svobodová, Bohumil Zálešák
Rok vydání: 2018
Předmět:
Zdroj: Archives of Dermatological Research. 310:413-424
ISSN: 1432-069X
0340-3696
DOI: 10.1007/s00403-018-1828-6
Popis: Exposure to solar radiation is a major cause of environmental human skin damage. The main constituent of solar UV light is UVA radiation (320-400 nm); however, the need for protection against UVA has been marginalized for a long time. As a result, there is still a lack of useful agents for UVA protection. In this study, the effect of silymarin (SM) and its main constituent silybin (SB) pre-treatment on UVA-stimulated damage to primary human dermal fibroblasts were carried out. The cells were pre-treated for 1 h with SB or SM and then were exposed to UVA light, using a solar simulator. The effect of SB and SM on reactive oxygen species (ROS) and glutathione (GSH) level, caspase-3 activity, single-strand breaks (SSB) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) was evaluated. Treatment with both SM and SB resulted in a reduction in UVA-stimulated ROS generation and SSB production, as well as in the prevention of GSH depletion, a decrease in the activation of caspase-3 and protein level of MMP-1. They also moderately increased HO-1 level and reduced HSP70 level. Our data showed that both SM and SB are non-phototoxic and have UVA-photoprotective potential and could be useful agents for UV-protective dermatological preparations.
Databáze: OpenAIRE
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