Differential long non-coding RNA expression profiles in human oocytes and cumulus cells
| Autor: | Said Assou, Julien Bouckenheimer, John De Vos, Céline Bruno, Charles-Henri Lecellier, Thérèse Commes, Jean-Marc Lemaitre, Patricia Fauque |
|---|---|
| Přispěvatelé: | Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), UFR des Sciences de Santé (Université de Bourgogne), Université de Bourgogne (UB), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Philips, Alexandre |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] lcsh:Medicine Reproductive technology Biology Real-Time Polymerase Chain Reaction Article Chromatin remodeling 03 medical and health sciences medicine Humans lcsh:Science Gene Metaphase MEG3 MALAT1 Cumulus Cells Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling lcsh:R Computational Biology Oocyte Long non-coding RNA Cell biology [SDV] Life Sciences [q-bio] Gene expression profiling 030104 developmental biology medicine.anatomical_structure Oocytes RNA Long Noncoding lcsh:Q |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2018, 8 (1), ⟨10.1038/s41598-018-20727-0⟩ Scientific Reports, 2018, 8 (1), ⟨10.1038/s41598-018-20727-0⟩ Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-20727-0⟩ |
| Popis: | Progress in assisted reproductive technologies strongly relies on understanding the regulation of the dialogue between oocyte and cumulus cells (CCs). Little is known about the role of long non-coding RNAs (lncRNAs) in the human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that were abundant in metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, and LINC01118) and CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, and VIM-AS1). These data were validated by RT-qPCR analysis using independent oocytes and CC samples. The functions of the identified lncRNAs were then predicted by constructing lncRNA-mRNA co-expression networks. This analysis suggested that MII oocyte lncRNAs could be involved in chromatin remodeling, cell pluripotency and in driving early embryonic development. CC lncRNAs were co-expressed with genes involved in apoptosis and extracellular matrix-related functions. A bioinformatic analysis of RNA-sequencing data to identify CC lncRNAs that are affected by maternal age showed that lncRNAs with age-related altered expression in CCs are essential for oocyte growth. This comprehensive analysis of lncRNAs expressed in human MII oocytes and CCs could provide biomarkers of oocyte quality for the development of non-invasive tests to identify embryos with high developmental potential. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|