Sputum versus nasopharyngeal samples for the molecular diagnosis of respiratory viral infection in cystic fibrosis: A pilot study

Autor: Hélène Le Guillou-Guillemette, Lisa Billard, S. Vallet, Emilie Cardot-Martin, Thierry Urban, Eric Farfour, Dominique Grenet, Geneviève Héry-Arnaud, Christopher Payan, Léa Pilorgé, Adissa Minoui-Tran, Jean Le Bihan, S. Ramel, Marie-Reine Munck, Françoise Troussier, Rozenn Le Berre
Přispěvatelé: Hôpital Foch [Suresnes], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Hémodynamique, Interaction Fibrose et Invasivité tumorales Hépatiques (HIFIH), Université d'Angers (UA), Génétique, génomique fonctionnelle et biotechnologies (UMR 1078) (GGB), EFS-Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Centre de Ressources et de Compétences de la Mucoviscidose [Roscoff] (CRCM), Société française de la mucoviscidose, PODEUR, Sophie
Rok vydání: 2021
Předmět:
Adult
Male
0301 basic medicine
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Adolescent
viruses
Concordance
Respiratory virus
Cystic fibrosis
Gastroenterology
Article
[SDU] Sciences of the Universe [physics]
03 medical and health sciences
fluids and secretions
0302 clinical medicine
Nasopharynx
Internal medicine
Multiplex polymerase chain reaction
medicine
Humans
Respiratory system
Child
Respiratory Tract Infections
Retrospective Studies
business.industry
nasopharyngeal
Respiratory viral infection
Sputum
Infant
Respiratory infection
Middle Aged
Multiplex molecular diagnosis
medicine.disease
respiratory tract diseases
030104 developmental biology
030228 respiratory system
[SDU]Sciences of the Universe [physics]
Child
Preschool
Pediatrics
Perinatology and Child Health
Feasibility Studies
Female
medicine.symptom
Influenza virus
business
Zdroj: Journal of Cystic Fibrosis
Journal of Cystic Fibrosis, 2021, 20 (3), pp.432-435. ⟨10.1016/j.jcf.2020.09.003⟩
ISSN: 1569-1993
DOI: 10.1016/j.jcf.2020.09.003
Popis: Highlights • First feasibility study of respiratory viral detection in Cystic Fibrosis sputum. • High concordance between sputum and nasopharyngeal samples in viral. • Multiplex-PCR performed in 30 paired samples: 83% viral congruency. • Sputum: an optimal non-invasive sample for viral diagnosis in cystic fibrosis.
Viruses are important agents in lung function deterioration in Cystic Fibrosis (CF). To date, no standard operating procedures (SOPs) have been established to determine which sampling method is the most effective for an optimal virological diagnosis of respiratory viral infections in CF. Here we investigated the performances of two sampling sites, sputum samples versus nasopharyngeal (NP) swabs, for thirty participants from three CF centres presenting an acute respiratory infection. Sputum and NP samples were simultaneously collected and multiplex PCR targeting 16 to 18 viruses were performed. Viruses were detected for 18/30 patients (60%). A high concordance between the sputum and NP samples was observed in 25 (83%) paired samples of which 13 tested positive and 12 tested negative. These results highlighted the relevance of sputum sampling for diagnostic of respiratory viruses in CF, which is less invasive and better accepted by CF patients than NP, and allows accurate bacterial detection.
Databáze: OpenAIRE
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