Pitanga (Eugenia uniflora L.) fruit juice and two major constituents thereof exhibit anti-inflammatory properties in human gingival and oral gum epithelial cells
| Autor: | Miriam Margit Ehrnhoefer-Ressler, Veronika Somoza, Jessica Walker, Julia Maria Imboeck, Marc Pignitter, Denise Josino Soares, Joel M. Walker, Isabella Montenegro Brasil |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
food.ingredient medicine.drug_class Syzygium Anti-Inflammatory Agents Gingiva engineering.material Tropical fruit Anti-inflammatory Cell Line Anthocyanins Beverages food Glucosides Healthy volunteers Botany medicine Humans RNA Messenger Interleukin 8 Food science Porphyromonas gingivalis Volatile Organic Compounds biology Chemistry Pulp (paper) Interleukin-8 Eugenia uniflora Epithelial Cells General Medicine Fibroblasts biology.organism_classification Fruit engineering Fruit juice Sesquiterpenes Food Science |
| Zdroj: | Food Funct.. 5:2981-2988 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/c4fo00509k |
| Popis: | Pitanga, Eugenia uniflora L., is a tropical fruit, which may be consumed as juice. While beneficial health effects of Eugenia uniflora L. leaf extracts have extensively been studied, limited data are available on an anti-inflammatory potential of pitanga juice. The aim of the presented study was to investigate anti-inflammatory properties of pitanga juice with regards to a prevention of inflammation-related periodontal diseases. For this purpose, six healthy volunteers swirled pitanga juice, containing 35% pitanga pulp, for 10 min. Thereafter, oral gum epithelial cells were harvested using a sterile brush and stimulated with lipopolysaccharides from Porphyromonas gingivalis (PG-LPS) for 6 h. Furthermore, human gingival fibroblasts (HGF-1) were used to elucidate the anti-inflammatory potential of pitanga juice constituents, cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one, in juice representative concentrations of 119 μg ml(-1) and 30 μg ml(-1), respectively. For the first time, an anti-inflammatory impact of pitanga juice on gingival epithelial cells was shown by means of an attenuation of IL-8 release by 55 ± 8.2% and 52 ± 11% in non-stimulated and PG-LPS-stimulated cells, respectively. In addition, both cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one reduced the LPS-stimulated CXCL8 mRNA expression by 50 ± 15% and 37 ± 18% and IL-8 release by 52 ± 9.9% and 45 ± 3.7% in HGF-1 cells, when concomitantly incubated with 10 μg ml(-1)PG-LPS for 6 h, revealing an anti-inflammatory potential of the volatile compound oxidoselina-1,3,7(11)-trien-8-one for the first time. |
| Databáze: | OpenAIRE |
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