Up regulation of ICAM-1 gene expression inhibits tumour growth and liver metastasis in colorectal carcinoma
| Autor: | Akiko Tachimori, Masaichi Ohira, Nobuya Yamada, Kosei Hirakawa, Hiroji Nishino, Kiyoshi Maeda, Masakazu Yashiro, Yoji Sakate |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Colorectal cancer Genetic enhancement Mice Nude Transfection Metastasis Viral vector Natural killer cell Adenoviridae Mice Cell Line Tumor Medicine Animals Humans Mice Inbred BALB C business.industry Liver Neoplasms medicine.disease Flow Cytometry Intercellular Adhesion Molecule-1 Up-Regulation medicine.anatomical_structure Oncology Cell culture Cancer cell Cancer research Leukocytes Mononuclear Female business Colorectal Neoplasms Cell Division Neoplasm Transplantation |
| Zdroj: | European journal of cancer (Oxford, England : 1990). 41(12) |
| ISSN: | 0959-8049 |
| Popis: | We have previously reported that decreased intercellular adhesion molecule-1 (ICAM-1) expression in cancer cells is associated with liver metastasis of colorectal cancer. In this study, we have investigated the effect of ICAM-1 gene transfection into the human colorectal cancer cell line LM-H3 on cell adhesiveness and cytotoxicity of peripheral blood mononuclear cells (PBMC) to cancer cells. Furthermore, we have investigated the effects of this gene transfer on subcutaneous tumour and liver metastases of LM-H3 in nude mice. More PBMC adhered to ICAM-1 transfected LM-H3 cells, LM-H3/ICAM-1, than to non-transfected LM-H3 cells and control LM-H3/Vector. Lysis of LM-H3/ICAM-1 cells by PBMC was significantly increased compared with LM-H3/Vector. Liver metastases with LM-H3/ICAM-1 cells were fewer in number and smaller than metastases with LM-H3/Vector. Intra-tumoural injection of ICAM-1 adenoviral vector significantly inhibited the growth of subcutaneous LM-H3 tumour. In conclusion ICAM-1 gene transfection using adenovirus vector might be an effective therapy for liver metastasis of colorectal carcinoma. |
| Databáze: | OpenAIRE |
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