Antihydatic and immunomodulatory effects of Algerian propolis ethanolic extract: In vitro and in vivo study
| Autor: | Dalila Mezioug, Nahla Deghbar, Touri Kahina, Chafia Touil Boukoffa, Yacine-Miloud Medjdoub |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962 030231 tropical medicine Spleen Pharmacology Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Oral administration parasitic diseases medicine antihydatic effect Echinococcus granulosus biology General Medicine Propolis biology.organism_classification In vitro cystic echinococcosis medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis immunomodulatory effect Tumor necrosis factor alpha algerian propolis |
| Zdroj: | Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 3, Pp 106-116 (2019) |
| ISSN: | 2352-4146 |
| Popis: | Objective: To evaluate the in vitro and in vivo effect of the Algerian propolis ethanolic extract (EEP) against Echinococcus granulosus (E. granulosus) infection. Methods: In vitro scolicidal activity of EEP was investigated on the protoscolices of hydatid cyst. This in vitro study was conducted by using an in vivo assay. BALB/c mice were inoculated with E. granulosus and treated with propolis for three months. Hydatid cysts development was assessed. Nitric oxide (NO), tumor necrosis factor alpha (TNF-α) production and inducible NO synthase, NF-κB, and TNF-α spleen expression were estimated by Griess method and immunofluorescence respectively. Results: Our study revealed that EEP has a high scolicidal activity against E. granulosus. Oral administration of EEP decreased TNF-α, NF-κB and inducible NO synthase expression in the spleen tissues in the CE+EEP group, in comparison with the CE group. Concomitantly, EEP treatment caused an important systemic decrease in NO and TNF-α levels. These findings are associated with the reduction of CE development. Conclusions: This is the first report demonstrating with interest the antihydatic and immunomodulatory effects of the Algerian EEP, suggesting its therapeutic potential for the hydatid disease treatment. |
| Databáze: | OpenAIRE |
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