LC–MS/MS measurement of endogenous oxytocin in the posterior pituitary and CSF of macaques: A pilot study
| Autor: | Tatiana A. Shnitko, David W. Erikson, Steven W. Blue, A. V. Kaucher |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Physiology Central nervous system Pilot Projects 030209 endocrinology & metabolism Endogeny Oxytocin Biochemistry Macaque Systemic circulation Article 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Endocrinology Tandem Mass Spectrometry Posterior pituitary biology.animal Internal medicine Lc ms ms Animals Medicine biology business.industry Macaca mulatta Peripheral medicine.anatomical_structure Pituitary Gland Models Animal Biological Assay business 030217 neurology & neurosurgery Chromatography Liquid medicine.drug |
| Zdroj: | Peptides |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2021.170544 |
| Popis: | Oxytocin (OT) is a nanopeptide released into systemic circulation via the posterior pituitary (peripheral) and into the central nervous system via widespread OTergic pathways (central). Central OT plays a significant role in variety of functions from social and executive cognition to immune regulation. Many ongoing studies explore its therapeutic potential for variety of neuropsychiatric disorders. Measures of peripheral OT levels are most frequently used as an indicator of its concentration in the central nervous system in humans and animal models. In this study, LC–MS/MS was used to measure OT in pituitary samples collected from adult male macaque monkeys in order to explore the correlation between individual levels of OT in the CSF (central) and pituitary (peripheral). We quantified individual differences in the levels of OT in the pituitaries (44−151 ng/mg) and CSF (41−66 pg/mL) of these monkeys. A positive correlation between these two measures was identified. These preliminary results allow for future analyses to determine whether LC–MS/MS measures of peripheral OT can be used as markers of OT levels in the brain of nonhuman primates that serve as valuable models for many human neuropsychiatric disorders. |
| Databáze: | OpenAIRE |
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