Aerobic turnover of dimethyl sulfide by the anoxygenic phototrophic bacterium thiocapsa roseopersicina
| Autor: | Michael Jansen, H.M. Jonkers, H. van Gemerden, van der Marc Maarel |
|---|---|
| Přispěvatelé: | Centraal Instituut voor Voedingsonderzoek TNO, Groningen Biomolecular Sciences and Biotechnology |
| Rok vydání: | 1999 |
| Předmět: |
Sulfide
purple sulfur bacterium Inorganic chemistry INHIBITION chemistry.chemical_element Electron donor Thiocapsa roseopersicina SULFUR-COMPOUNDS Dimethylsulfoniopropionate OXIDATION Biochemistry Microbiology DEMETHYLATION chemistry.chemical_compound PHYTOPLANKTON Genetics Sulfate-reducing bacteria dimethylsulfoniopropionate dimethyl sulfide Molecular Biology Nutrition chemistry.chemical_classification MICROBIAL MAT Strain (chemistry) Chemistry fungi CONSUMPTION General Medicine DIMETHYLSULFOXIDE Sulfur Anoxygenic photosynthesis SULFATE-REDUCING BACTERIA DIMETHYLSULFONIOPROPIONATE DMSP Dimethyl sulfide Nuclear chemistry |
| Zdroj: | Archives of Microbiology, 3, 172, 150-156 Archives of Microbiology, 172(3), 150-156. SPRINGER |
| ISSN: | 1432-072X 0302-8933 |
| Popis: | This is the first report describing the complete oxidation of dimethyl sulfide (DMS) to sulfate by an anoxygenic, phototrophic purple sulfur bacterium. Com- plete DMS oxidation was observed in cultures of Thio- capsa roseopersicina M11 incubated under oxic/light conditions, resulting in a yield of 30.1 mg protein mmol -1 . No oxidation of DMS occurred under anoxic/light condi- tions. Chloroform, methyl butyl ether, and 3-amino-1,2,4- triazole, which are specific inhibitors of aerobic DMS ox- idation in thiobacilli and hyphomicrobia, did not affect DMS oxidation in strain M11. This could be due to lim- ited transport of the inhibitors through the cell membrane. The growth yield on sulfide as sole electron donor was 22.2 mg protein mmol -1 under anoxic/light conditions. Since aerobic respiration of sulfide would have resulted in yields lower than 22 mg protein mmol -1 , the higher yield on DMS under oxic/light conditions suggests that the methyl groups of DMS have served as an additional car- bon source or as an electron donor in addition to the sul- fide moiety. The kinetic parameters V max and Km for DMS oxidation under oxic/light conditions were 12.4 ± 1.3 nmol (mg protein) -1 min -1 and 2 μM, respectively. T. roseoper- sicina M11 also produced DMS by cleavage of dimethyl- sulfoniopropionate (DMSP). Specific DMSP cleavage rates increased with increasing initial substrate concentra- tions, suggesting that DMSP lyase was only partly induced at lower initial DMSP concentrations. A comparison of T. roseopersicina strains revealed that only strain M11 was able to oxidize DMS and cleave DMSP. Both strain M11 and strain 5811 accumulated DMSP intracellularly during growth, while strain 1711 showed neither of these charac- teristics. Phylogenetic comparison based on 16S rRNA gene sequence revealed a similarity of 99.0% between strain M11 and strain 5811, and 97.6% between strain M11 and strain 1711. DMS and DMSP utilization thus ap- pear to be strain-specific. |
| Databáze: | OpenAIRE |
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