Heterogeneity of Genetic Damage in Cervical Nuclei and Lymphocytes in Women with Different Levels of Dysplasia and Cancer-Associated Risk Factors
| Autor: | Carlos Alvarez-Moya, Hugo Castañeda-Vázquez, José O. Chavez-Chavez, Alfredo Feria-Velasco, Alejandro A. Canales-Aguirre, Mónica Reynoso-Silva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Article Subject DNA damage Uterine Cervical Neoplasms lcsh:Medicine Biology General Biochemistry Genetics and Molecular Biology Genetic Heterogeneity Risk Factors Uterine Cervical Dysplasia medicine Humans Lymphocytes Cervical cancer Cell Nucleus General Immunology and Microbiology Genetic heterogeneity lcsh:R Cancer General Medicine medicine.disease Neoplasm Proteins Comet assay Cell nucleus medicine.anatomical_structure Dysplasia Female Research Article DNA Damage |
| Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2015/293408 |
| Popis: | The comet assay can be used to assess genetic damage, but heterogeneity in the length of the tails is frequently observed. The aims of this study were to evaluate genetic damage and heterogeneity in the cervical nuclei and lymphocytes from patients with different levels of dysplasia and to determine the risk factors associated with the development of cervical cancer. The study included 97 females who presented with different levels of dysplasia. A comet assay was performed in peripheral blood lymphocytes and cervical epithelial cells. Significant genetic damage (P≤0.05) was observed only in patients diagnosed with nuclei cervical from dysplasia III (NCDIII) and lymphocytes from dysplasia I (LDI). However, the standard deviations of the tail lengths in the cervical nuclei and lymphocytes from patients with dysplasia I were significantly different (P≤0.0001) from the standard deviations of the tail lengths in the nuclei cervical and lymphocytes from patients with DII and DIII (NCDII, NCDIII and LDII, LDIII), indicating a high heterogeneity in tail length. Results suggest that genetic damage could be widely present but only manifested as increased tail length in certain cell populations. This heterogeneity could obscure the statistical significance of the genetic damage. |
| Databáze: | OpenAIRE |
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