Platelet-Derived Growth Factor Gene Delivery Stimulatesex VivoGingival Repair
| Autor: | William V. Giannobile, Qi Ming Jin, Sarah A. Webb, Orasa Anusaksathien |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Time Factors
Platelet-derived growth factor medicine.medical_treatment Genetic Vectors Gingiva Biology Gene delivery medicine.disease_cause Article Adenoviridae chemistry.chemical_compound Gene expression medicine Humans Regeneration Platelet-Derived Growth Factor Tissue Engineering Growth factor Regeneration (biology) Gene Transfer Techniques General Engineering Proto-Oncogene Proteins c-sis Fibroblasts Molecular biology chemistry Cancer research biology.protein Collagen Ex vivo Platelet-derived growth factor receptor |
| Zdroj: | Tissue Engineering. 9:745-756 |
| ISSN: | 1557-8690 1076-3279 |
| DOI: | 10.1089/107632703768247421 |
| Popis: | Destruction of tooth support due to the chronic inflammatory disease periodontitis is a major cause of tooth loss. There are limitations with available treatment options to tissue engineer soft tissue periodontal defects. The exogenous application of growth factors (GFs) such as platelet-derived growth factor (PDGF) has shown promise to enhance oral and periodontal tissue regeneration. However, the topical administration of GFs has not led to clinically significant improvements in tissue regeneration because of problems in maintaining therapeutic protein levels at the defect site. The utilization of PDGF gene transfer may circumvent many of the limitations with protein delivery to soft tissue wounds. The objective of this study was to test the effect of PDGF-A and PDGF-B gene transfer to human gingival fibroblasts (HGFs) on ex vivo repair in three-dimensional collagen lattices. HGFs were transduced with adenovirus encoding PDGF-A and PDGF-B genes. Defect fill of bilayer collagen gels was measured by image analysis of cell repopulation into the gingival defects. The modulation of gene expression at the defect site and periphery was measured by RT-PCR during a 10-day time course after gene delivery. The results demonstrated that PDGF-B gene transfer stimulated potent (>4-fold) increases in cell repopulation and defect fill above that of PDGF-A and corresponding controls. PDGF-A and PDGF-B gene expression was maintained for at least 10 days. PDGF gene transfer upregulated the expression of phosphatidylinosital 3-kinase and integrin alpha5 subunit at 5 days after adenovirus transduction. These results suggest that PDGF gene transfer has potential for periodontal soft tissue-engineering applications. |
| Databáze: | OpenAIRE |
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