Analysis ofpolIntegrase Sequences in Diverse HIV Type 1 Strains Using a Prototype Genotyping Assay
| Autor: | John Hackett, Sarah E. Hudelson, Sushil G. Devare, Vera Holzmayer, Priscilla Swanson, Natalia Marlowe, Peter Lawrence Smith, Susan H. Eshleman |
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| Rok vydání: | 2009 |
| Předmět: |
Genotype
Molecular Sequence Data Immunology Argentina Mutation Missense Sequence Homology HIV Infections HIV Integrase Genetic analysis Virus South Africa Virology Drug Resistance Viral medicine Cluster Analysis Humans Uganda Cameroon HIV Integrase Inhibitors Genotyping Phylogeny Genetics biology Elvitegravir virus diseases Sequence Notes Sequence Analysis DNA Thailand biology.organism_classification Raltegravir Integrase Infectious Diseases Lentivirus HIV-1 biology.protein Brazil medicine.drug |
| Zdroj: | AIDS Research and Human Retroviruses. 25:343-345 |
| ISSN: | 1931-8405 0889-2229 |
| DOI: | 10.1089/aid.2008.0236 |
| Popis: | A prototype assay was used to genotype integrase (IN) from 120 HIV-1- infected IN inhibitor-naive adults from Argentina, Brazil, Cameroon, South Africa, Thailand, and Uganda. Subtype designations based on analysis of pol IN sequences were A (14), B (15), C (12), D (11), F (12), G (7), H (1), CRF01_AE (9), CRF02_AG (34), CRF22_01A1 (4), and CRF37_cpx (1). Ten (8.3%) of 120 samples had mutations associated with reduced susceptibility to the IN inhibitors, raltegravir and elvitegravir. Two samples had E92Q (both subtype B) and eight had E157Q (2A, 1C, 1D, 1F, 3 CRF02_AG). Some samples had other mutations selected by these drugs including T97A, and some had amino acid polymorphisms at positions associated with raltegravir and elvitegravir resistance. Mutations associated with other investigational HIV IN inhibitors were also identified. This suggests that HIV strains may vary in their natural susceptibility to HIV IN inhibitors. |
| Databáze: | OpenAIRE |
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