Expression and regulation of glucocorticoid-induced leucine zipper in the developing anterior pituitary gland
| Autor: | Tom E. Porter, Stefanie A Malkiewicz, Laura E. Ellestad, G.R. Welch, H. David Guthrie |
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| Rok vydání: | 2008 |
| Předmět: |
Pituitary gland
medicine.medical_specialty Leucine zipper Pro-Opiomelanocortin Molecular Sequence Data Chick Embryo Biology chemistry.chemical_compound Endocrinology Anterior pituitary Downregulation and upregulation Pituitary Gland Anterior Corticosterone Internal medicine Gene expression medicine Animals Humans Amino Acid Sequence RNA Messenger 3' Untranslated Regions Molecular Biology Cells Cultured Leucine Zippers Sequence Homology Amino Acid Embryogenesis Gene Expression Regulation Developmental Prolactin Up-Regulation medicine.anatomical_structure chemistry Follicle Stimulating Hormone beta Subunit Chickens Transcription Factors Hormone |
| Zdroj: | Journal of Molecular Endocrinology. 42:171-183 |
| ISSN: | 1479-6813 0952-5041 |
| Popis: | The expression profile of glucocorticoid-induced leucine zipper (GILZ) in the anterior pituitary during the second half of embryonic development in the chick is consistent with in vivo regulation by circulating corticosteroids. However, nothing else has been reported about the presence of GILZ in the neuroendocrine system. We sought to characterize expression and regulation of GILZ in the chicken embryonic pituitary gland and determine the effect of GILZ overexpression on anterior pituitary hormone levels. Pituitary GILZ mRNA levels increased during embryogenesis to a maximum on the day of hatch, and decreased through the first week after hatch. GILZ expression was rapidly upregulated by corticosterone in embryonic pituitary cells. To determine whether GILZ regulates hormone gene expression in the developing anterior pituitary, we overexpressed GILZ in embryonic pituitary cells and measured mRNA for the major pituitary hormones. Exogenous GILZ increased prolactin mRNA above basal levels, but not as high as that in corticosterone-treated cells, indicating that GILZ may play a small role in lactotroph differentiation. The largest effect we observed was a twofold increase in FSH β subunit in cells transfected with GILZ but not treated with corticosterone, suggesting that GILZ may positively regulate gonadotroph development in a manner not involving glucocorticoids. In conclusion, this is the first report to characterize avian GILZ and examine its regulation in the developing neuroendocrine system. We have shown that GILZ is upregulated by glucocorticoids in the embryonic pituitary gland and may regulate expression of several pituitary hormones. |
| Databáze: | OpenAIRE |
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