Clastogenic inosine nucleotide as components of the chromosome breakage factor in scleroderma patients
| Autor: | Christian Auclair, Alain Gouyette, Ingrid Emerit, Arlette Levy |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Biophysics
Biochemistry Mass Spectrometry Superoxide dismutase Clastogen Inosine Diphosphate Reference Values medicine Humans Inosine Triphosphate Nucleotide Lymphocytes Inosine Inosine Nucleotides Molecular Biology Cells Cultured Chromatography High Pressure Liquid Chromosome Aberrations chemistry.chemical_classification Scleroderma Systemic biology Superoxide Dismutase Chemistry Ribonucleotides Cell culture biology.protein Chromatid Chromosome breakage Sister Chromatid Exchange Mutagens medicine.drug |
| Zdroj: | Archives of Biochemistry and Biophysics. 278:238-244 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(90)90253-u |
| Popis: | In the present study, we attempted to identify the chemical nature of the clastogenic factor (CF) from patients with progressive systemic sclerosis (scleroderma). Computerized mass spectrometry of clastogenic fractions obtained by HPLC of plasma ultrafiltrates detected molecular peaks compatible with inosine triphosphate and inosine diphosphate (ITP and IDP). The concomitant detection of IDP, together with ITP, and the absence of these peaks in nonclastogenic fractions and corresponding control fractions are arguments in favor of a biological relevance of these observations. The most important confirmation came from the clastogenic effect of commercial ITP and IDP added to the culture medium of the test cultures. The induction of chromatid type damage by these substances in lymphocytes exposed in the G0 phase of their cell cycle and the prevention of this damage by superoxide dismutase are analogous to the observations with CF. |
| Databáze: | OpenAIRE |
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