Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
| Autor: | Tania García-Mendiola, Patrick Kung, Jose Luis Pau, Alicia de Andrés, Sourav Garg, Encarnación Lorenzo, Andrés Redondo-Cubero, Sandra Cortijo-Campos, M. Briones, Sergio Catalán-Gómez |
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| Přispěvatelé: | UAM. Departamento de Física Aplicada, UAM. Departamento de Química Analítica y Análisis Instrumental, Electrónica y Semiconductores (EXP C-032), Ministerio de Economía y Competitividad (España), Comunidad de Madrid |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Photoluminescence lcsh:Medicine 02 engineering and technology 010402 general chemistry Epitaxy 01 natural sciences Breast cancer Monolayer lcsh:Science Multidisciplinary Nanoscale materials urogenital system business.industry Hybridization probe lcsh:R Física Química 021001 nanoscience & nanotechnology 0104 chemical sciences DNA and RNA Sapphire Optical biosensors Optoelectronics lcsh:Q Trion 0210 nano-technology business Luminescence Biosensor MoS2 monolayer Biomarkers |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) Digital.CSIC. Repositorio Institucional del CSIC instname Biblos-e Archivo. Repositorio Institucional de la UAM |
| ISSN: | 2045-2322 |
| Popis: | The following article appeared in Scientific Reports 10 (2020): 16039 and may be found at https://www.nature.com/articles/s41598-020-73029-9 In this work we report on the characterization and biological functionalization of 2D MoS2 fakes, epitaxially grown on sapphire, to develop an optical biosensor for the breast cancer biomarker miRNA21. The MoS2 fakes were modifed with a thiolated DNA probe complementary to the target biomarker. Based on the photoluminescence of MoS2, the hybridization events were analyzed for the target (miRNA21c) and the control non-complementary sequence (miRNA21nc). A specifc redshift was observed for the hybridization with miRNA21c, but not for the control, demonstrating the biomarker recognition via PL. The homogeneity of these MoS2 platforms was verifed with microscopic maps. The detailed spectroscopic analysis of the spectra reveals changes in the trion to excitation ratio, being the redshift after the hybridization ascribed to both peaks. The results demonstrate the benefts of optical biosensors based on MoS2 monolayer for future commercial devices The research is supported by the MINECO (CTQ2017-84309-C2-2-R, CTQ2017-84309-C2-1-R, ELECTROBIONET, RED2018-102412-T) and Comunidad de Madrid (TRANSNANOAVANSENS, P2018/NMT4349) projects. ARC acknowledges Ramón y Cajal program (under contract number RYC-2015-18047) |
| Databáze: | OpenAIRE |
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