Resonance Raman detection of antioxidants using an iron oxide nanoparticle catalysed decolourisation assay
| Autor: | Duncan Graham, Sian Sloan-Dennison, Neil C. Shand, Karen Faulds |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Antioxidant
medicine.medical_treatment Inorganic chemistry Ascorbic Acid 02 engineering and technology 010402 general chemistry Ferric Compounds 01 natural sciences Biochemistry Antioxidants Analytical Chemistry Catalysis chemistry.chemical_compound symbols.namesake Chlorogenic acid Electrochemistry medicine Environmental Chemistry Benzothiazoles Spectroscopy Detection limit ABTS Substrate (chemistry) 021001 nanoscience & nanotechnology Ascorbic acid Glutathione 0104 chemical sciences QD450 chemistry symbols Nanoparticles Chlorogenic Acid Sulfonic Acids 0210 nano-technology Raman spectroscopy |
| Zdroj: | The Analyst. 142:4715-4720 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c7an01151b |
| Popis: | Nanozymes are metal nanoparticles with catalytic properties that can be used to oxidise peroxidase substrates giving a colorimetric response which can be detected using UV-vis, and recently, Raman spectroscopy. Due to their ease of synthesis and increased stability, nanozymes are being increasing investigated to replace conventional enzymes for the detection of biomolecules. Here we exploit the catalytic activity of iron oxide (Fe2O3) nanoparticles combined with the substrate 2,2-Azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) in a decolourisation assay for the detection of antioxidants. Fe2O3 nanoparticles were used to catalyse the oxidation of ABTS to its green radical cation which, upon the addition of an antioxidant, resulted in a decolourisation due to the reduction of the radical cation caused by the hydrogen donating antioxidant. The assay was applied for the detection of multiple antioxidants (glutathione, chlorogenic acid and ascorbic acid), and was followed by monitoring the resonance Raman scattering from the ABTS solution using a portable Raman system with 785 nm laser excitation. This novel assay has the potential to be optimised to detect antioxidant activity in body fluid with low limits of detection with point of use monitoring. |
| Databáze: | OpenAIRE |
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