High-Level Multiplex DNA Amplification
| Autor: | Natalia E. Broude, Charles R. Cantor, Kristina Driscoll |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pharmacology
Base Sequence Multiple displacement amplification Recombinase Polymerase Amplification DNA Amplicon Biology Molecular Inversion Probe Polymerase Chain Reaction Polymorphism Single Nucleotide Molecular biology Primer dimer Genetics Chromosomes Human Humans Multiplex ligation-dependent probe amplification Applications of PCR Hot start PCR DNA Primers |
| Zdroj: | Antisense and Nucleic Acid Drug Development. 11:327-332 |
| ISSN: | 1087-2906 |
| DOI: | 10.1089/108729001753231704 |
| Popis: | We present data on efficient amplification of large number of DNA targets using a single-tube polymerase chain reaction (PCR). This is a further enhancement of our approach to multiplexed PCR based on PCR suppression, which allows multiple DNA amplification using only one sequence-specific primer per amplicon while the second primer is common for all targets (Broude, N.E., et al., Proc. Natl. Acad. Sci. USA 98, 206-211, 2001). The reaction conditions have been optimized for simultaneous synthesis of 30 DNA targets, mostly consisting of fragments containing single nucleotide polymorphisms (SNP). The size of the amplified fragments, derived from many different human chromosomes, varies from 100 to 600 bp. We conclude that this method has potential for highly multiplexed DNA amplification useful for SNP analyses, DNA diagnostics, and forensics. |
| Databáze: | OpenAIRE |
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