A product of phosphatidylinositol-3 kinase is elevated in dividing HT29 colonic epithelial cells
| Autor: | Gustav J. Dobos, Xiao Rong Wu, Alexis Traynor-Kaplan |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
G2 Phase
PI-3 kinase Phosphatidylinositol 3 phosphate Cell division Biophysics Mitosis Cell cycle Biology Phosphatidylinositols Biochemistry Phosphatidylinositol 3-Kinases chemistry.chemical_compound HT29 Cells Phosphatidylinositol Phosphates Structural Biology Colonic epithelia Cell Adhesion Tumor Cells Cultured Genetics Humans Phosphatidylinositol Molecular Biology Cell growth Kinase Phosphatidylinositol 3-phosphate Phosphotransferases Cell Biology Cell biology chemistry Colonic Neoplasms Cell Division |
| Zdroj: | FEBS Letters. 324:143-146 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(93)81381-9 |
| Popis: | Phosphatidylinositol 3 kinase (PI-3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in phosphoinositide metabolism during the cell cycle of HT-29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separated into mitotic and quiescent populations and their phospholipid composition was analyzed. Radiolabelled phospholipids from cells labelled with 32PO4 or [3H]myoinositol were analyzed by TLC and/or deacylated and analyzed by HPLC. In all cases, levels of phosphatidyl-inositol 3 phosphate from mitotic phase cells were double that in resting populations. Therefore, levels of a product of PI-3 kinase are elevated and may play a role in cell division. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text