Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model
| Autor: | Bojana Obradovic, Lisa E. Freed, Jerry H. Meldon, Gordana Vunjak-Novakovic |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
0303 health sciences
Environmental Engineering Chemistry General Chemical Engineering 0206 medical engineering Type II collagen 02 engineering and technology Matrix (biology) Chondrogenesis 020601 biomedical engineering Chondrocyte Glycosaminoglycan Extracellular matrix 03 medical and health sciences Tissue culture medicine.anatomical_structure Bioreactor medicine 030304 developmental biology Biotechnology Biomedical engineering |
| Zdroj: | AICHE Journal |
| ISSN: | 1547-5905 0001-1541 |
| DOI: | 10.1002/aic.690460914 |
| Popis: | Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time. |
| Databáze: | OpenAIRE |
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