Human amniotic membrane stem cells’ conditioned medium has better support for in‐vitro production of bovine embryos than FBS
| Autor: | Ali Shadmanesh, Ebrahim Ahmadi, Hassan Nazari, Naser Shams-Esfandabadi, Abolfazl Shirazi |
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| Rok vydání: | 2021 |
| Předmět: |
Fertilization in Vitro
Embryo Culture Techniques Andrology Endocrinology medicine Animals Humans Inner cell mass Amnion Blastocyst Hatching Chemistry Stem Cells Proteins Embryo Oocyte In vitro Culture Media medicine.anatomical_structure Membrane Culture Media Conditioned embryonic structures Cattle Animal Science and Zoology Stem cell Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 57:173-184 |
| ISSN: | 1439-0531 0936-6768 |
| Popis: | Apart from oocyte quality, the media used has a significant effect on the production and quality of blastocysts produced in vitro. This study was designed to evaluate the replacement of serum with human amniotic membrane stem cells' conditioned medium (hAMSCs-CM) during bovine embryo culture on the quantity and quality of produced blastocysts. The in-vitro-produced embryos on the third day of IVC were randomly divided into the following culture groups: SOFaa + 5% FBS (Control), SOFaa + 5% hAMSCs-CM (5% CM), SOFaa + 2.5% hAMSCs-CM + 2.5% FBS (2.5% CM) and SOFaa + hAMSC co-culture (co-culture). The blastocyst and hatching rates, blastocyst cells number (the number of trophectoderm, inner cell mass and total cells), and the expression of some developmentally important genes (OCT4, PLAC8 and COX2 genes) in the treated groups, especially in the 5% CM, compared to the control had improved (p |
| Databáze: | OpenAIRE |
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