Strain dependent effects of prenatal stress on gene expression in the rat hippocampus
| Autor: | James I. Koenig, Sherry Leonard, Eric W. Neeley, Ralph Berger |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty alpha7 Nicotinic Acetylcholine Receptor Offspring Radioimmunoassay Hippocampus Experimental and Cognitive Psychology Receptors Nicotinic Receptors N-Methyl-D-Aspartate Article Rats Sprague-Dawley Behavioral Neuroscience Receptors Glucocorticoid Glucocorticoid receptor Species Specificity Adrenal Cortex Hormones Pregnancy Neurotrophic factors Internal medicine Gene expression medicine Animals RNA Messenger Brain-derived neurotrophic factor biology Tumor Necrosis Factor-alpha Brain-Derived Neurotrophic Factor Age Factors Gene Expression Regulation Developmental Rats Endocrinology Animals Newborn Prenatal stress Rats Inbred Lew Prenatal Exposure Delayed Effects biology.protein Female GRIN2B Stress Psychological |
| Zdroj: | Physiology & Behavior. 104:334-339 |
| ISSN: | 0031-9384 |
| DOI: | 10.1016/j.physbeh.2011.02.032 |
| Popis: | Multiple animal models have been developed to recapitulate phenotypes of the human disease, schizophrenia. A model that simulates many of the cognitive and sensory deficits of the disorder is the use of random variable prenatal stress (PS) in the rat. These deficits suggest a molecular origin in the hippocampus, a brain region that plays a role in the regulation of stress. To study both hippocampal gene expression changes in offspring of prenatally stressed dams and to address genetic variability, we used a random array of prenatal stressors in three different rat strains with diverse responses to stress: Fischer, Sprague–Dawley, and Lewis rats. Candidate genes involved in stress, schizophrenia, cognition, neurotrophic effects, and immunity were selected for assessment by real-time quantitative PCR under resting conditions and following a brief exposure to restraint stress. PS resulted in significant differences in gene expression in the offspring that were strain dependent. mRNA expression for the N-methyl- d -aspartate receptor subtype 2B ( Grin2b ) was increased, and tumor necrosis factor-alpha ( Tnfα ) transcript was decreased in PS Sprague–Dawley and Lewis rats, but not in the Fischer rats. Expression of brain-derived neurotrophic factor ( Bdnf ) mRNA in the hippocampus was increased after an acute stress in all controls of each strain, yet a decrease was seen after acute stress in the PS Sprague–Dawley and Lewis rats. Expression of the glucocorticoid receptor ( Nr3c1 ) was decreased in the Fischer strain when compared to Lewis or Sprague–Dawley rats, though the Fischer rats had markedly higher α7 nicotinic receptor ( Chrna7 ) expression. The expression differences seen in these animals may be important elements of the phenotypic differences seen due to PS and genetic background. |
| Databáze: | OpenAIRE |
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