Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation
| Autor: | Erol Rüştü Bozkurt, Esma Konuk, Ercument Ovali, Murat Yener, Mehmet Yilmaz, Harun Cansiz, Necdet Demir, Mehmet Veli Karaaltin, Yetkin Zeki Yilmaz, Fatma Eyüpoğlu, Aysegul Batioglu-Karaaltin, Ergun Bozdag, Ozgur Yigit |
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| Přispěvatelé: | Acibadem University Dspace, İÜC, Cerrahpaşa Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü |
| Rok vydání: | 2018 |
| Předmět: |
lcsh:Medicine
Matrix (biology) Glycosaminoglycan Scaffold 03 medical and health sciences chemistry.chemical_compound Freeze-drying 0302 clinical medicine Tissue engineering Medicine 030223 otorhinolaryngology Decellularization Tissue Engineering business.industry lcsh:R Deoxycholic acid lcsh:Otorhinolaryngology lcsh:RF1-547 Lactic acid Transplantation Trachea Freeze Drying Otorhinolaryngology chemistry 030220 oncology & carcinogenesis Surgery Original Article business Biomedical engineering Deoxycholic Acid |
| Zdroj: | Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology, Vol 12, Iss 1, Pp 86-94 (2019) |
| ISSN: | 1976-8710 |
| Popis: | YILMAZ, Yetkin Zeki/0000-0002-5734-9751; Bozdag, Ergun/0000-0001-7412-1129; Batioglu-Karaaltin, Aysegul/0000-0002-8620-4375 WOS:000454984200012 PubMed ID: 30326701 Objectives. The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. Methods. Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5 +/- 0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up. Results. DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dl, and 53 mg/L). Conclusion. Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time. Istanbul University Scientific Research Project Unit (BAP) [398688]; Istanbul, Turkey This study was supported by Istanbul University Scientific Research Project Unit (BAP, project no. 398688), Istanbul, Turkey. |
| Databáze: | OpenAIRE |
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