Increased Expression of Cyclooxygenase-2 to -1 in Human Colorectal Cancers and Adenomas, but not in Hyperplastic Polyps
| Autor: | Mineko Ushiama, Satoshi Miyazaki, Kokichi Sugano, Hisanao Ohkura, Hajime Sano, Masato Maekawa, Tadao Kakizoe, Takuji Gotoda, Shin Fujita, Takao Sekiya, Toshihiro Yokota |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adenoma
Adult Male Cancer Research Pathology medicine.medical_specialty Colorectal cancer Molecular Sequence Data Colonic Polyps Polymerase Chain Reaction Intestinal mucosa medicine Humans Radiology Nuclear Medicine and imaging Amino Acid Sequence RNA Messenger Intestinal Mucosa Polymorphism Single-Stranded Conformational Aged Aged 80 and over Messenger RNA biology Rectal Neoplasms business.industry Mucous membrane General Medicine Middle Aged medicine.disease Immunohistochemistry medicine.anatomical_structure Oncology Hyperplastic Polyp Prostaglandin-Endoperoxide Synthases Colonic Neoplasms biology.protein Female Cyclooxygenase business |
| Zdroj: | Japanese Journal of Clinical Oncology. 28:421-426 |
| ISSN: | 1465-3621 0368-2811 |
| DOI: | 10.1093/jjco/28.7.421 |
| Popis: | Background: Non-steroidal anti-inflammatory drugs can reduce the risk of colorectal cancer. Reportedly, mRNA expression of cyclooxygenase-2 (COX-2) is elevated in human colorectal cancers compared with accompanying normal mucosa. The present study was undertaken to establish a simple analytical procedure to quantify COX-2 expression levels and to characterize CQX-2 expression levels in humancolorectal cancers, adenomas and hyperplastic polyps. Methods: The combination of PCR using common primers designed in the highly conserved regions and fluorescence-based single-strand conformation polymorphism (F-SSCP) analysis of the products is used for quantitative determination of the proportions of COX-2 mRNA in human colorectal cancers, adenomas, hyperplasticpolyps and accompanying normal mucosa. Results: The present F-SSCP analysis was a simple and powerful method for quantitative determination of the proportions of COX-2 mRNA. The proportion of COX-2 mRNA was higher in cancer tissues than in accompanying normal mucosa in 46 of the 50 cancers. There was no significant correlation betweenthe increaseof the COX-2proportion and tumor location or stages. The enhanced COX-2expression was also observed in colorectaladenomas. On the other hand, the proportion of COX-2 mRNA in hyperplastic polyps was not significantly differentfrom that in normal mucosa. Conclusions: The proportion of COX-2 to COX-1 expression was elevated in most human colorectal cancers and adenomas, but not in hyperplastic polyps. Therefore, the increased proportion of COX-2expression mightbe an early event in thecarcinogenesis of colorectal cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|