Molecular characterization of human adenoviruses associated with respiratory infection in Turkey
| Autor: | Tuba Çiğdem Oğuzoğlu, Bahattin Taylan Koç, Hürrem Bodur, Aliye Bastug, Ayşe Başak Altaş, Gulay Korukluoglu, Fatma Bayrakdar |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male medicine.medical_specialty Genotype Turkey Sequence analysis Polymerase Chain Reaction Pathology and Forensic Medicine Adenovirus Infections Human 03 medical and health sciences 0302 clinical medicine Risk Factors Nasopharynx Epidemiology medicine Immunology and Allergy Humans Respiratory system Child Pathogen Respiratory Tract Infections Tropism Phylogeny Molecular Epidemiology biology Adenoviruses Human virus diseases Respiratory infection Genetic Variation Infant General Medicine Sequence Analysis DNA Middle Aged biology.organism_classification Virology eye diseases Mastadenovirus 030104 developmental biology 030220 oncology & carcinogenesis Child Preschool Female |
| Zdroj: | APMIS : acta pathologica, microbiologica, et immunologica ScandinavicaReferences. 129(1) |
| ISSN: | 1600-0463 |
| Popis: | Human adenoviruses (HAdVs) are responsible for various clinical diseases. Molecular epidemiological studies of respiratory HAdVs are limited in Turkey. To determine the main genotypes and epidemiological characteristics of HAdVs in patients with respiratory symptoms. HAdV PCR-positive extracts of nasal/nasopharyngeal specimens sent to the Turkish Public Health Institution from various cities of Turkey in 2015-2016 were investigated by seminested PCR. Partial sequence analysis of the hexon gene of HAdVs was performed. SPSSv.24.0 was used. A total of 23/68 (33.82%) HAdV-positive samples were amplified. Mastadenovirus B, C, D, and F were detected and mastadenovirus B (10/23; 43.5%) and C (10/23; 43.5%) were predominant strains. Interestingly, HAdV-F known to have gastrointestinal system tropism was detected in two patients with respiratory symptoms. HAdV-B3 was the most prevalent genotype (9/23; 39.1%). Also, HAdV-B7 is defined as a reemerging pathogen. It is noteworthy that there is a cluster of four HAdV-C strains showing a close paraphyletic relationship with HAdV-2/6 intertypic recombination. To our knowledge, this is the first study showing that HAdV-B7 reemerging pathogen circulating in patients with respiratory infections in our country. It is also necessary to emphasize that HAdV-2/6 recombinant strains were detected in this study for the first time in Turkey. |
| Databáze: | OpenAIRE |
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