Bacillus salidurans sp. nov., isolated from salt-accumulated pepper rhizospheric soil
| Autor: | Jin-Soo Son, Ye-Ji Hwang, Sa-Youl Ghim, Soo-Yeong Lee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial Salinity Bacillus Diamino acid Peptidoglycan Sodium Chloride Diaminopimelic Acid 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Cell Wall RNA Ribosomal 16S Botany Pepper Republic of Korea Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology Bacillus (shape) Base Composition biology Phylogenetic tree Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Phosphatidylglycerols Vitamin K 2 General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology chemistry Capsicum Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 69(1) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea. Cell growth of strain KNUC7312T occurred at 10–45 °C (optimum, 30 °C) and pH 7–12 (optimum, pH 7). In addition, this strain was able to tolerate 0–12 % NaCl (w/v) concentration (optimum, 0–1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318T (98.0 %). The predominant respiratory quinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0. The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid. Strain KNUC7312T showed a low DNA–DNA relatedness value (47.36 %) with B. humi DSM 16318T, which supported that this strain represents a novel Bacillus species. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312T represents a novel species within the genera Bacillus . The name Bacillus salildurans sp. nov. is proposed. The type strain is KNUC7312T (KCTC 33852T=CGMCC 1.13629T). |
| Databáze: | OpenAIRE |
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