Chitosan-Stabilized Selenium Nanoparticles and Metformin Synergistically Rescue Testicular Oxidative Damage and Steroidogenesis-Related Genes Dysregulation in High-Fat Diet/Streptozotocin-Induced Diabetic Rats
| Autor: | Safaa I. Khater, Mohamed A. Nassan, Mohamed M.M. Metwally, Amany Abdel-Rahman Mohamed, Ahmed Hamed Arisha, Manal Ewaiss Hassan, Yasmina M. Abd El-Hakim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system endocrine system diseases Physiology type 2 diabetes mellitus Clinical Biochemistry 030209 endocrinology & metabolism medicine.disease_cause Biochemistry Article male fertility Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound steroidogenesis related genes 0302 clinical medicine testicular dysfunction Internal medicine medicine oxidative stress Molecular Biology Steroidogenic acute regulatory protein lcsh:RM1-950 nutritional and metabolic diseases Cell Biology chitosan-stabilized selenium nanoparticles Malondialdehyde Streptozotocin Sperm Metformin 030104 developmental biology Endocrinology lcsh:Therapeutics. Pharmacology chemistry metformin Oxidative stress medicine.drug Hormone |
| Zdroj: | Antioxidants Volume 10 Issue 1 Antioxidants, Vol 10, Iss 17, p 17 (2021) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox10010017 |
| Popis: | Background: this study examined the metformin (MF) and/or chitosan stabilized selenium nanoparticles (CH-SeNPs) efficacy to alleviate the male reproductive function impairment in a high-fat diet feed with low-dose streptozotocin (HFD/STZ) induced type 2 diabetes mellitus (T2DM) diabetic rat model. Methods: control non-diabetic, HFD/STZ diabetic, HFD/STZ+MF, HFD/STZ+CH-SeNPs, and HFD/STZ+MF+CH-SeNPs rat groups were used. After 60 days, semen evaluation, hormonal assay, enzymatic antioxidant, lipid peroxidation, testis histopathology, and the steroidogenesis-related genes mRNA expressions were assessed. Results: in the HFD/STZ diabetic rats, sperm count and motility, male sexual hormones, and testicular antioxidant enzymes were significantly reduced. However, sperm abnormalities and testicular malondialdehyde were significantly incremented. The steroidogenesis-related genes, including steroidogenic acute regulatory protein (StAr), cytochrome11A1 (CYP11A1), cytochrome17A1 (CYP17A1), and hydroxysteroid 17-beta dehydrogenase 3 (HSD17B3), and the mitochondrial biogenesis related genes, including peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC&alpha ) and sirtuin (SIRT), were significantly downregulated in the HFD/STZ diabetic rats. However, CYP19A1mRNA expression was significantly upregulated. In contrast, MF and/or CH-SeNPs oral dosing significantly rescued the T2DM-induced sperm abnormalities, reduced sperm motility, diminished sexual hormones level, testicular oxidative damage, and steroidogenesis-related genes dysregulation. In the MF and CH-SeNP co-treated group, many of the estimated parameters differ considerably from single MF or CH-SeNPs treated groups. Conclusions: the MF and CH-SeNPs combined treatment could efficiently limit the diabetic complications largely than monotherapeutic approach and they could be considered a hopeful treatment option in the T2DM. |
| Databáze: | OpenAIRE |
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