Evaluation of oxidative stress and antioxidant status, serum trace mineral levels and cholinesterases activity in cattle infected with Anaplasma marginale

Autor: Awat Samiei, Abbas Imani-Baran, Farhad Farhang-Pajuh, Mousa Tavassoli, Nasser Hajipour, Bijan Esmaeilnejad
Rok vydání: 2018
Předmět:
0301 basic medicine
Serum
Anaplasmosis
Antioxidant
Erythrocytes
medicine.medical_treatment
Iran
medicine.disease_cause
Parasitemia
Antioxidants
0403 veterinary science
Lipid peroxidation
chemistry.chemical_compound
Malondialdehyde
Cholinesterases
Butyrylcholinesterase
chemistry.chemical_classification
biology
Chemistry
Glutathione peroxidase
Anemia
04 agricultural and veterinary sciences
Catalase
Anaplasma marginale
Infectious Diseases
Acetylcholinesterase
medicine.medical_specialty
040301 veterinary sciences
Cattle Diseases
Glucosephosphate Dehydrogenase
Microbiology
Superoxide dismutase
03 medical and health sciences
Internal medicine
medicine
Animals
Cholinesterase
Glutathione Peroxidase
Superoxide Dismutase
Acetylcholine
Trace Elements
Osmotic Fragility
Oxidative Stress
030104 developmental biology
Endocrinology
biology.protein
Erythrocyte Count
Cattle
Lipid Peroxidation
Oxidative stress
Biomarkers
Zdroj: Microbial pathogenesis. 123
ISSN: 1096-1208
Popis: This study was undertaken to assess the influence of an Anaplasma marginale infection on oxidative stress and antioxidant status, trace elements and cholinesterase as markers of the inflammatory process and biomarkers of oxidative imbalance. An infected group comprised of 35 crossbred Holstein cattle, about 2-3 years old, naturally infected with Anaplasma marginale, were divided into 4 subgroups according to their parasitemia rates (1%, 1-10%, 10-20%,20%) and also 10 healthy cattle as control were selected. Blood samples were taken and hematological parameters, activities of antioxidant enzymes including erythrocyte glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), glucose-6-phosphate dehydrogenase (G6PD), total antioxidant capacity (TAC), median corpuscularfragility (MCF) as well as acetylcholinesterase (AChE), and serum concentrations of antioxidant trace minerals (copper, iron, zinc, manganese, and selenium) and butyrylcholinesterase (BChE) were determined. In addition, as an index of lipid peroxidation, the level of malondialdehyde (MDA) was measured. The results revealed a significant decrease (P 0.05) in RBC count, packed cell volume (PCV) and Hb concentration as well as the activities of erythrocyte GSH-Px, SOD, CAT, G6PD, TAC, MCF and AChE and serum concentrations of Cu, Zn, Mn, Se and BchE in the infected cattle. In contrast, significantly increased (P 0.05) levels of MDA and erythrocyte osmotic fragility as well as serum concentration of iron were recorded in the infected animals. The significant decrease in antioxidant enzyme activities and substantial elevated levels of lipid peroxidation and erythrocyte osmotic fragility associated with the notable increase in parasitemia indicate increased exposure of RBCs to oxidative damage. Furthermore, decrease of cholinesterase in infection by A. marginale can and directly or indirectly lead to increase acetylcholine levels potent anti-inflammatory molecules, thereby inhibiting inflammation.
Databáze: OpenAIRE
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