Evaluation of porB PCR-Amplicon Restriction Endonuclease Analysis as a Method To Determine porB Variable-Region Sequences in Nonserotypeable Meningococci
| Autor: | Diana R. Martin, Kristin H. Dyet, Robin S. Simmonds |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Molecular Sequence Data Porins Neisseria meningitidis medicine.disease_cause Polymerase Chain Reaction law.invention Disease Outbreaks law medicine Humans Amino Acid Sequence Serotyping Polymerase chain reaction biology Genetic Variation Reproducibility of Results Bacteriology DNA Restriction Enzymes Sequence Analysis DNA Amplicon biology.organism_classification Virology Bacterial Typing Techniques Meningococcal Infections Restriction enzyme Neisseriaceae New Zealand |
| Popis: | porB PCR-amplicon restriction endonuclease analysis is a rapid, simple method developed to assess porB variation in nonserotypeable meningococci isolated during New Zealand's epidemic of meningococcal disease. Most nonserotypeable meningococci isolated between 1990 and 1999 inclusively either were type 4 (40.5%) or contained the porB variable region 1 (VR1)-19, VR2-D, VR3-7, and VR4-14a sequences (45.1%). |
| Databáze: | OpenAIRE |
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