Nitric Oxide-Mediated Apoptosis in Murine Mastocytoma
| Autor: | Toshihiro Nakajima, Ko-ichi Kawahara, Takami Matsuyama, Yasuko Soejima, Ikuro Maruyama, Isao Kitajima |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cell Survival
Biophysics Gene Expression Mast-Cell Sarcoma Apoptosis Biology Arginine Nitric Oxide Biochemistry Dexamethasone Cell Line Nitric oxide Mice chemistry.chemical_compound Tumor Cells Cultured medicine Animals RNA Messenger Molecular Biology omega-N-Methylarginine Dose-Response Relationship Drug Tumor Necrosis Factor-alpha Mastocytoma DNA Neoplasm Cell Biology medicine.disease Molecular biology Nitric oxide synthase Microscopy Electron chemistry Enzyme Induction Mast cell sarcoma biology.protein Omega-N-Methylarginine DNA fragmentation Tumor necrosis factor alpha Amino Acid Oxidoreductases Nitric Oxide Synthase Cell Division |
| Zdroj: | Biochemical and Biophysical Research Communications. 204:244-251 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.2451 |
| Popis: | To investigate how the number of mast cells is controlled, we studied a murine mastocytoma cell line. Based on electron microscopic observation of nuclear condensation and electrophoretic evidence with DNA fragmentation, these mastocytoma wells were shown to undergo apoptosis. This apoptosis was dependent on the concentrations of serum and L-arginine and was enhanced by TNF-alpha. We confirmed that apoptosis was mediated by nitric oxide (NO) synthase; inducible NO synthase (iNOS) mRNA was strongly expressed in apoptotic cells, while an inhibitor of NOS, NG-monomethyl-L-arginine, and dexamethasone prevented apoptosis in addition to inhibiting iNOS mRNA expression. Our results suggest that iNOS expression is very important in regulating the proliferation of mast cells under pathological conditions. |
| Databáze: | OpenAIRE |
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