A microELISA system for the detection of both anti-HIV-1 and anti-HIV-2 antibodies
| Autor: | J.A. Hellings, H.J. Theunissen, E.D. Sprengers, P. B. W. Ten Kortenaar |
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| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
biology medicine.diagnostic_test Anti hiv Immunology virus diseases Enzyme-Linked Immunosorbent Assay Peptide HIV Antibodies Sensitivity and Specificity Virology Molecular biology Virus Epitope Viral envelope Antigen chemistry Immunoassay HIV-2 HIV-1 biology.protein medicine Humans Immunology and Allergy Antibody |
| Zdroj: | Journal of Immunological Methods. 139:77-82 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(91)90353-h |
| Popis: | Current anti-HIV-1 screening tests combine an excellent anti-HIV-1 sensitivity with a sensitivity of only 28–93% for anti-HIV-2 positive plasma or serum samples. The reactivity of anti-HIV-2 sera in anti-HIV-1 screening tests is based mainly on the immunological cross-reactivity of the GAG and POL proteins of HIV-1 and HIV-2. We describe here a sandwich immunoassay, in which HIV-1 viral lysate is combined with an HIV-2 ENV synthetic peptide, corresponding to the immunodominant envelope epitope, as the coating antigens on microELISA plates. This immunoassay has a sensitivity of 100% for anti-HIV-1 (128 sera tested) and 100% for anti-HIV-2 (109 sera tested). Assay specificity with fresh human donor sera was 99.9% (2256 sera tested). |
| Databáze: | OpenAIRE |
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