ytiB and ythA Genes Reduce the Uranium Removal Capacity of Bacillus atrophaeus
| Autor: | Shiqi Xiao, Faqin Dong, Shanqiang Wang, Yunlai Tang, Chao Wang, Shilin Chen, Li Wang, Xiaoming Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cytochrome Transgene 030106 microbiology gene ythA Catalysis Article Microbiology Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Plasmid Carbonic anhydrase Gene ytiB uranium contamination Bacillus atrophaeus Physical and Theoretical Chemistry Molecular Biology Gene lcsh:QH301-705.5 Spectroscopy biology Strain (chemistry) Chemistry microbial remediation Organic Chemistry General Medicine biology.organism_classification Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 biology.protein Bacteria |
| Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 7, p 1766 (2019) International Journal of Molecular Sciences Volume 20 Issue 7 |
| ISSN: | 1422-0067 |
| Popis: | Two Bacillus atrophaeus strains, the first being a highly stress-resistant ATCC 9372 strain and the Ua strain identified from a chromium mine by our lab, differ in their abilities to tolerate and remove Uranium (VI) from contaminated water. An increase in U(VI) concentration in growth media led to a decrease in the tolerance and bio-remedial capacity of both strains. However, under high concentrations of U(VI) in the growth media, the ATCC 9372 strain demonstrated a higher tolerance and a higher removal capacity than the Ua strain. Two approaches, transcriptome sequencing and transgenic technology, were used to elucidate the relationship between particular genes within these two strains and their U(VI) removal capacity. Sequencing confirmed the expression of two genes unique to the Ua strain, previously designated ytiB and ythA. They encode putative proteins that show the highest levels of identity to carbonic anhydrase and cytochrome bd terminal oxidase I, respectively. Using the pBE-S DNA vector, ytiB and ythA were transformed into the ATCC 9372 strain of Bacillus atrophaeus. Under a U(VI) concentration of 120 mg/L, the removal rates of the transgenic ATCC 9372-ytiB and ATCC 9372-ythA strains decreased by 7.55% and 7.43%, respectively, compared to the removal rate of the control strain transformed with empty plasmid. The results suggest that both ythA and ytiB genes have a negative influence on the uranium removing capacity of Bacillus atrophaeus. This finding will help to elucidate the molecular mechanisms of uranium removal by bacteria. |
| Databáze: | OpenAIRE |
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