Total fractionation and characterization of the water-soluble polysaccharides isolated from Enteromorpha intestinalis
| Autor: | Hao Zaibin, Xiong Feng, Liu Yufeng, Li Xia, Liu Feifei, Huiying Chen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DPPH
02 engineering and technology Fractionation Uronic acid Chemical Fractionation Polysaccharide 01 natural sciences Biochemistry Antioxidants Sepharose Ulva chemistry.chemical_compound Polysaccharides Structural Biology Monosaccharide Molecular Biology chemistry.chemical_classification Chromatography Hydroxyl Radical Sulfates 010405 organic chemistry Free Radical Scavengers General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Molecular Weight chemistry Hydroxyl radical 0210 nano-technology Chromatography column |
| Zdroj: | International Journal of Biological Macromolecules. 111:319-325 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.01.018 |
| Popis: | Water-soluble crude polysaccharides (WE) were extracted from the Enteromorpha intestinalis with hot water and further fractionated on a DEAE-52 Cellulose chromatography column and Sepharose CL-6B gel-permeation chromatography to afford one neutral fraction (WE-11) and five acidic fractions (WE-21, WE-31, WE-32, WE-41 and WE-42). Monosaccharide analysis showed that WE-32, WE-41 and WE-42 were all composed of Rha and GlcA, WE-21 and WE-31 contained Man and Rha, and WE-11 was composed of GlcA and Xyl. The molecular weight of WE-11, WE-21, WE-31 WE-32, WE-41 and WE-42 were 2.23 × 105, 1.42 × 105, 1.42 × 105, 2.62 × 104, 2.23 × 105 and 8.09 × 104, respectively. Then, antioxidant activities in vitro were investigated on the basis of DPPH radical assay, reducing power assay, hydroxyl radical assay and superoxide radical assay. Of all polysaccharides fractions, WE-32 and WE-41 had relative higher content of sulfate and GlcA. In addition, WE-32 and WE-41 showed relative stronger antioxidant activity and inhibitory activity in vitro. The antioxidant activities of polysaccharides were not a function of a single factor but a combination of several factors, such as monosaccharide composition, molecular weights, protein content, uronic acid and sulfate content. |
| Databáze: | OpenAIRE |
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