Development of site-specific antibody-conjugated immunoliposomes for sensitive detection of disease biomarkers
| Autor: | Xiao-Wei Sun, Xiao-Yi Gao, Jin-Bao Tang, Hong-Ming Yang, Xiao-Kun Zhang, Xi-Feng Sun, Meng-Ran Li |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Nanoscale. 13:17648-17654 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/d1nr04659d |
| Popis: | Liposome-based immunoassay (LIA) is an attractive protocol for amplifying the detection signals because of the excellent ability of liposomes to encapsulate signal marker compounds. The antigen-binding activity of the conjugated antibodies on the liposomal surface is crucial for the specificity and sensitivity of LIA. We present here a general platform to ensure that antibodies can conjugate onto the surface of liposomes in a site-specific and oriented manner. A His-handle-modified antibody with Fc region-specific and covalent conjugation was first fabricated using a photoactivatable ZBpa-His tag that was engineered using the aminoacyl-tRNA synthetase/suppressor tRNA technique. Based on the high affinity between the His tag and divalent metal ions, the novel His-modified antibody was oriented onto the surface of nickel ion-modified liposomes encapsulating horseradish peroxidase. With the prostate-specific antigen as a model, the detection efficiency of the new immunoliposomes was evaluated by chemiluminescence immunoassay. The immunoliposomes exhibited a limit of detection of 0.2 pg mL−1, which was a six time improvement compared with that of the chemical-coupled antibody–liposome conjugates. Thus, the proposed immunoliposomes are expected to hold potential applications for the sensitive detection of various biomarkers in complicated serum samples. |
| Databáze: | OpenAIRE |
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